Replication of Theiler's murine encephalomyelitis virus in Xenopus laevis oocytes

被引:1
|
作者
Franco, D
Stevens, I
Steurbaut, S
Goris, J
Rombaut, B
机构
[1] Vrije Univ Brussels, Dept Microbiol & Hyg, B-1090 Brussels, Belgium
[2] Katholieke Univ Leuven, Afdeling Biochem, Fac Geneeskunde, B-3000 Louvain, Belgium
关键词
virus replication; oocytes; theilovirus;
D O I
10.1016/j.virusres.2004.06.009
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Xenopus laevis oocytes call be Used as ail alternative system to study replication of Theiler's murine encephalomyelitis virus (TMEV). We have shown that transcript RNA, containing full-length viral genome. can be directly used to programme the oocytes. In the programmed oocytes, there is correct viral translation, polyprotein processing and assembly of capsid proteins leading to the production of infectious TMEV. The vast majority of de novo synthesised virions were found in the medium in which the programmed oocytes. were incubated and not in the oocytes. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:35 / 38
页数:4
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