Demethyleneberberine promotes apoptosis and suppresses TGF-β/Smads induced EMT in the colon cancer cells HCT-116

被引：20
|
作者：
Wang, Zehao [1 ,2 ]
Wang, Lixia [1 ,2 ]
Shi, Boya [1 ,2 ]
Sun, Xiuli [1 ,2 ]
Xie, Yinrong [1 ,2 ]
Yang, Haonan [1 ,2 ]
Zi, Chengting [1 ,3 ]
Wang, Xuanjun [1 ,3 ]
Sheng, Jun [1 ]
机构：
[1] Yunnan Agr Univ, Minist Educ, Key Lab Pu Er Tea Sci, Kunming 650201, Yunnan, Peoples R China
[2] Yunnan Agr Univ, Coll Food Sci & Technol, Kunming, Yunnan, Peoples R China
[3] Yunnan Agr Univ, Coll Sci, Kunming, Yunnan, Peoples R China
来源：
CELL BIOCHEMISTRY AND FUNCTION | 2021年 / 39卷 / 06期
关键词：
apoptosis; cell cycle; demethyleneberberine; epidermal‐ mesenchymal transition; transforming growth factor‐ β EPITHELIAL-MESENCHYMAL-TRANSITION; GROWTH-FACTOR; REPRESSOR; DISEASE; BETA; MICE;
D O I：
10.1002/cbf.3638
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Colorectal cancer (CRC) is one of the most common malignant tumours in the world. Recent reports have revealed natural products displayed inhibition on colon cancer potential by suppressing transforming growth factor-beta/Smads induced epidermal-mesenchymal transition (EMT). In this article, 12 kinds of natural berberine analogues were screened for their effects on the inhibition of the colon cancer cells, the results showed that demethyleneberberine (DM-BBR) exhibited an interesting and potential effect on inducing the apoptosis of HCT-116 cells with drug concentrations of 6, 12 and 18 mu M. Particularly, DM-BBR reversed the EMT process by inhibiting the expression of p-Smad2 and p-Smad3 in the transforming growth factor-beta/Smads signal pathway, up-regulated pro-apoptotic protein cleaved caspase-9, and blocked cell cycle at the S phase and increasing the expression of cyclin proteins P27 and P21. Taken together, these findings suggested that DM-BBR could promote apoptosis and suppress TGF-beta/Smads induced EMT in the colon cancer cells HCT-116.
引用
收藏
页码：763 / 770
页数：8
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