Clonal Expansion of Sulfonamide Resistant Escherichia coli Isolates Recovered from Diarrheic Calves

被引：0
|
作者：
Adnan, Muhammad [1 ]
Khan, Hamayun [2 ]
Kashif, Jam [1 ]
Ahmad, Shakoor [2 ]
Gohar, Ali [3 ]
Ali, Amjad [4 ]
Khan, Muhammad Atif [1 ]
Shah, Said Sajjad Ali [2 ]
Hassan, Mohammad Farooque [7 ]
Irshad, Muhammad [5 ]
Khan, Nazir Ahmad [6 ]
Rahman, Sadeeq Ur [3 ]
机构：
[1] Sindh Agr Univ Tando Jam, Dept Pharmacol, Tandojam, Pakistan
[2] Univ Agr Peshawar, Dept Anim Hlth, Peshawar, Pakistan
[3] Abdul Wali Khan Univ, Coll Vet Sci & AH, Mardan, Pakistan
[4] Hazara Univ, Ctr Human Genet, Mansehra, Pakistan
[5] Aljouf Univ, Dept Prevent Dent, Sakakah, Saudi Arabia
[6] Univ Agr, Dept Nutr, Peshawar, Pakistan
[7] Shaheed Benazir Bhutto Univ Vet & Anim Sci, Sakrand Sindh, Pakistan
来源：
PAKISTAN VETERINARY JOURNAL | 2017年 / 37卷 / 02期
关键词：
Diarrhea; E; coli; Minimum inhibitory concentration; Sul genes; Sulfadimidine resistance; GENES; SUL1;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A total of 30 Escherichia coli isolates from 50 random samples of diarrheic calves were screened against resistance to sulfonamides. Minimum inhibitory concentration (MIC) by microdilution method indicated that 25/30 (83.3%) were found resistant to sulfadimidine, 27/30 (90%) were resistant to sulfadiazine, while 22/30 (73.3%) were found resistant to sulfamethaxole. These phenotypically sulfonamide resistant isolates were then probed for the presence of Sul genes (Sul-1-3) by polymerase chain reaction (PCR) using specific primers. PCR results revealed that 23/25, 25/27 and 20/22 isolates carried Sul-1 gene, respectively. Interestingly, all these isolates were found negative for the presence of Sul-2 and Sul-3 genes. Of note, no Sul genes could be verified in 02 phenotypically resistant isolates. The abundance of Sul1 gene and absence of Sul-2 and Sul-3 genes indicating a clonal expansion of sulfonamide resistant E. coli that might be linked to excessive abuse of sulfonamides in animals. (C) 2017 PVJ. All rights reserved
引用
收藏
页码：230 / 232
页数：3
相关论文
共 50 条
  • [21] Comparative pharmacodynamics of ciprofloxacin, enrofloxacin and norfloxacin for Escherichia coli of diarrheic calves
    Sidhu, P.
    Beri, S.
    Kaur, G.
    Rampal, S.
    JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 2015, 38 : 13 - 13
  • [22] Molecular characterization of Escherichia coli isolates recovered from broilers with cellulitis
    Mueller, Anja
    Bernd, Kathrin Schulze
    Seinige, Diana
    Braun, Ann-Sophie
    Kumm, Franziska
    Kehrenberg, Corinna
    POULTRY SCIENCE, 2024, 103 (06) : 1 - 7
  • [23] Molecular characterization of antibiotic resistant Escherichia coli isolates recovered from food samples and outpatient Clinics, KSA
    Hemeg, Hassan A.
    SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2018, 25 (05) : 928 - 931
  • [24] Characterization of virulence factors and phylogenetic group determination of Escherichia coli isolated from diarrheic and non-diarrheic calves from Brazil
    Fernanda Morcatti Coura
    Soraia de Araújo Diniz
    Jamili Maria Suhet Mussi
    Marcos Xavier Silva
    Andrey Pereira Lage
    Marcos Bryan Heinemann
    Folia Microbiologica, 2017, 62 : 139 - 144
  • [25] Characterization of virulence factors and phylogenetic group determination of Escherichia coli isolated from diarrheic and non-diarrheic calves from Brazil
    Coura, Fernanda Morcatti
    Diniz, Soraia de Araujo
    Suhet Mussi, Jamili Maria
    Silva, Marcos Xavier
    Lage, Andrey Pereira
    Heinemann, Marcos Bryan
    FOLIA MICROBIOLOGICA, 2017, 62 (02) : 139 - 144
  • [26] Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970
    Van Bost, S
    Bâbe, MH
    Jacquemin, E
    Mainil, J
    VETERINARY MICROBIOLOGY, 2001, 82 (04) : 311 - 320
  • [27] The characterisation of antimicrobial resistant Escherichia coli from dairy calves
    Mwenifumbo, Merning
    Cookson, Adrian L.
    Zhao, Shengguo
    Fayaz, Ahmed
    Browne, A. Springer
    Benschop, Jackie
    Burgess, Sara A.
    JOURNAL OF MEDICAL MICROBIOLOGY, 2023, 72 (08)
  • [28] Subtypes of intimin among non-toxigenic Escherichia coli from diarrheic calves in Brazil
    Aidar, L
    Penteado, AS
    Trabulsi, LR
    Blanco, JE
    Blanco, M
    Blanco, J
    de Castro, AFP
    CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 2000, 64 (01): : 15 - 20
  • [29] Genes associated with Escherichia coli isolates from calves with diarrhoea and/or septicaemia
    Wu, G.
    Mafura, M.
    Carter, B.
    Lynch, K.
    Anjum, M. F.
    Woodward, M. J.
    Pritchard, G. C.
    VETERINARY RECORD, 2010, 166 (22) : 691 - 692
  • [30] Occurrence and characterisation of enterohaemorrhagic Escherichia coli isolates from diarrhoeic calves
    Badouei M.A.
    Salehi T.Z.
    Khorasgani M.R.
    Tadjbakhsh H.
    Brujeni G.N.
    Comparative Clinical Pathology, 2010, 19 (3) : 295 - 300
12345