The effect of the frictional heat on retention and efficiency in thermostated or insulated chromatographic columns packed with sub-2-μm particles

被引：7
|
作者：
Lambert, Nandor [1 ]
Felinger, Attila [1 ,2 ,3 ,4 ]
机构：
[1] MTA PTE Mol Interact Separat Sci Res Grp, Ifjusag Utja 6, H-7624 Pecs, Hungary
[2] Univ Pecs, Dept Analyt & Environm Chem, Ifjusag Utja 6, H-7624 Pecs, Hungary
[3] Univ Pecs, Szentagothai Res Ctr, Ifjusag Utja 6, H-7624 Pecs, Hungary
[4] Univ Pecs, Inst Bioanal, Med Sch, Szigeti Ut 12, H-7624 Pecs, Hungary
来源：
JOURNAL OF CHROMATOGRAPHY A | 2018年 / 1565卷
关键词：
UHPLC; Column efficiency; Frictional heat; Sub-2-mu m particles; Thermostating; Insulation; PRESSURE LIQUID-CHROMATOGRAPHY; MASS-TRANSFER KINETICS; PARKING-MOMENT ANALYSIS; TEMPERATURE-GRADIENTS; CORE-SHELL; MU-M; PERFORMANCE; PHASE; DISSIPATION; STRATEGY;
D O I：
10.1016/j.chroma.2018.06.038
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The mass-transfer properties of a core-shell packing material with 1.6 mu m particle diameter, and that of a fully porous packing material with 1.7 mu m particle diameter were investigated and compared. The first absolute and the second central moments of the peaks of the homologous series of alkylbenzenes, over a wide range of mobile phase velocities were measured and used for the calculation of the mass transfer coefficients. For the evaluation of the band broadening caused by the thermal dissimilarities, the measurements were carried out under thermostated conditions and also at near adiabatic insulation of the columns. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：89 / 95
页数：7
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