Lysyl Oxidase (Lox) Gene Deficiency Affects Osteoblastic Phenotype

被引：54

作者：

Pischon, N. ^{[1
]}

Maki, J. M. ^{[2
]}

Weisshaupt, P. ^{[1
]}

Heng, N. ^{[1
]}

Palamakumbura, A. H. ^{[3
]}

N'Guessan, P. ^{[4
]}

Ding, A. ^{[5
]}

Radlanski, R. ^{[5
]}

Renz, H. ^{[5
]}

Bronckers, T. A. L. J. J. ^{[6
]}

Myllyharju, J. ^{[2
]}

Kielbassa, A. M. ^{[1
]}

Kleber, B. M. ^{[1
]}

Bernimoulin, J. -P. ^{[1
]}

Trackman, P. C. ^{[3
]}

机构：

[1] Charite Univ Med Berlin, Univ Sch Dent Med, ChariteCtr 3, Dept Operat Dent & Periodontol, D-14197 Berlin, Germany

[2] Univ Oulu, Dept Med Biochem & Mol Biol, Bioctr Oulu, Oulu Ctr Cell Matrix Res, Oulu, Finland

[3] Boston Univ, Goldman Sch Dent Med, Dept Oral Biol & Periodontol, Boston, MA 02215 USA

[4] Charite, Dept Infect Dis & Pulmonol, D-13353 Berlin, Germany

[5] Charite, Dept Expt Dent Med, D-13353 Berlin, Germany

[6] Vrije Univ Amsterdam, ACTA, Dept Oral Cell Biol, Amsterdam, Netherlands

来源：

CALCIFIED TISSUE INTERNATIONAL | 2009年 / 85卷 / 02期

关键词：

Lysyl oxidase; LOXL1-4; Knockout; Osteoblast; TUMOR-SUPPRESSOR ACTIVITY; COLLAGEN CROSS-LINKING; BONE; PROTEIN; APOPTOSIS; EXPRESSION; CLONING; MATRIX; MINERALIZATION; INACTIVATION;

D O I：

10.1007/s00223-009-9252-8

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Lysyl oxidase (LOX) catalyzes cross-linking of elastin and collagen, which is essential for the structural integrity and function of bone tissue. The present study examined the role of Lox gene deficiency for the osteoblast phenotype in primary calvarial osteoblasts from E18.5 Lox knockout (Lox (-/-) ) and wild type (wt) (C57BL/6) mice. Next to Lox gene depletion, mRNA expression of Lox isoforms, LOXL1-4, was significantly downregulated in Lox (-/-) bone tissue. A significant decrease of DNA synthesis of Lox (-/-) osteoblasts compared to wt was found. Early stages of osteoblastic apoptosis studied by annexin-V binding as well as later stages of DNA fragmentation were not affected. However, mineral nodule formation and osteoblastic differentiation were markedly decreased, as revealed by significant downregulation of osteoblastic markers, type I collagen, bone sialoprotein, and Runx2/Cbfa1.

引用

页码：119 / 126

页数：8

共 50 条

[41] Lysyl oxidase (lox) is essential for maintaining the integrity of elastic fibres in various tissues
Mäki, JM
Räsäsen, J
Tikkanen, H
Sormunen, R
Mäkikallio, K
Myllyharju, J
Kivirikko, KI
Soininen, R
MATRIX BIOLOGY, 2004, 23 (06) : 407 - 407
[42] Lysyl Oxidase (LOX) Expression in Colorectal Cancer - Exploration of Potential Clinical Value
Sansom, L.
Hutchins, G. G. A.
Shires, M.
Hemmings, G.
Tinkler-Hundal, E.
Grabsch, H.
Quirke, P.
JOURNAL OF PATHOLOGY, 2012, 228 : S26 - S26
[43] The effects of suramin on lysyl oxidase (LOX), telomerase activity and the differentiation of osteosarcoma cells
Buchinger, B.
Varga, F.
Spitzer, S.
Klaushofer, K.
CALCIFIED TISSUE INTERNATIONAL, 2007, 80 : S73 - S73
[44] Lysyl oxidase (LOX) may serve as a promising therapeutic target for lung cancer
Katsuda, Masahito
Muraki, Nao
Kawabe, Nozomi
Tomatsu, Aya
Yoshida, Mikina
Ohashi, Ayano
Umeda, Kanna
Tanaka, Ichidai
Morise, Masahiro
Matsushima, Miyoko
Kawabe, Tsutomu
Sato, Mitsuo
RESPIROLOGY, 2024, 29 : 35 - 35
[45] LYSYL OXIDASE (LOX) IN VASCULAR REMODELLING: INSIGHT FROM A NEW ANIMAL MODEL
Orriols, M.
Guadall, A.
Galan, M.
Rodriguez-Calvo, R.
Marti, I.
Briones, A. M.
Varona, S.
Navarro, M. A.
de Diego, A.
Osada, J.
Martinez-Gonzalez, J.
Rodriguez, C.
ATHEROSCLEROSIS, 2014, 235 (02) : E43 - E43
[46] Effect of lysyl oxidase (LOX) on corpus cavernous fibrosis caused by ischaemic priapism
Gao, Liang
Wu, Changjing
Fu, Fudong
You, Xuanhe
Ma, Xue
Qin, Feng
Li, Tao
Wang, Run
Yuan, Jiuhong
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2018, 22 (03) : 2018 - 2022
[47] Exploring the role of copper-dependent lysyl oxidase (LOX) in metastatic growth
Jasmer-McDonald, Kimberly
Petris, Michael
Shanbhag, Vinit
Gudekar, Nikita
CANCER RESEARCH, 2017, 77
[48] IDENTIFICATION OF A LYSYL OXIDASE-RELATED GENE
MELLOTT, JK
WATKINS, JR
YANG, TP
FASEB JOURNAL, 1993, 7 (07): : A1306 - A1306
[49] Targeting lysyl oxidase (LOX) overcomes chemotherapy resistance in triple negative breast cancer
Ozge Saatci
Aysegul Kaymak
Umar Raza
Pelin G. Ersan
Ozge Akbulut
Carolyn E. Banister
Vitali Sikirzhytski
Unal Metin Tokat
Gamze Aykut
Suhail A. Ansari
Hayriye Tatli Dogan
Mehmet Dogan
Pouria Jandaghi
Aynur Isik
Fatma Gundogdu
Kemal Kosemehmetoglu
Omer Dizdar
Sercan Aksoy
Aytekin Akyol
Aysegul Uner
Phillip J. Buckhaults
Yasser Riazalhosseini
Ozgur Sahin
Nature Communications, 11
[50] Lysyl Oxidase (LOX)-Dependent Matrix Stiffening Contributes to Diabetic Retinal Endothelial Inflammation
Yang, Xiao
Scott, Harry
Xu, Jun
Ardekani, Soroush
Cabrera, Andrea
Mohideen, Umar
Ghosh, Kaustabh
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2015, 56 (07)

← 1 2 3 4 5 →