Lysyl Oxidase (Lox) Gene Deficiency Affects Osteoblastic Phenotype

被引：54

作者：

Pischon, N. ^{[1
]}

Maki, J. M. ^{[2
]}

Weisshaupt, P. ^{[1
]}

Heng, N. ^{[1
]}

Palamakumbura, A. H. ^{[3
]}

N'Guessan, P. ^{[4
]}

Ding, A. ^{[5
]}

Radlanski, R. ^{[5
]}

Renz, H. ^{[5
]}

Bronckers, T. A. L. J. J. ^{[6
]}

Myllyharju, J. ^{[2
]}

Kielbassa, A. M. ^{[1
]}

Kleber, B. M. ^{[1
]}

Bernimoulin, J. -P. ^{[1
]}

Trackman, P. C. ^{[3
]}

机构：

[1] Charite Univ Med Berlin, Univ Sch Dent Med, ChariteCtr 3, Dept Operat Dent & Periodontol, D-14197 Berlin, Germany

[2] Univ Oulu, Dept Med Biochem & Mol Biol, Bioctr Oulu, Oulu Ctr Cell Matrix Res, Oulu, Finland

[3] Boston Univ, Goldman Sch Dent Med, Dept Oral Biol & Periodontol, Boston, MA 02215 USA

[4] Charite, Dept Infect Dis & Pulmonol, D-13353 Berlin, Germany

[5] Charite, Dept Expt Dent Med, D-13353 Berlin, Germany

[6] Vrije Univ Amsterdam, ACTA, Dept Oral Cell Biol, Amsterdam, Netherlands

来源：

CALCIFIED TISSUE INTERNATIONAL | 2009年 / 85卷 / 02期

关键词：

Lysyl oxidase; LOXL1-4; Knockout; Osteoblast; TUMOR-SUPPRESSOR ACTIVITY; COLLAGEN CROSS-LINKING; BONE; PROTEIN; APOPTOSIS; EXPRESSION; CLONING; MATRIX; MINERALIZATION; INACTIVATION;

D O I：

10.1007/s00223-009-9252-8

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Lysyl oxidase (LOX) catalyzes cross-linking of elastin and collagen, which is essential for the structural integrity and function of bone tissue. The present study examined the role of Lox gene deficiency for the osteoblast phenotype in primary calvarial osteoblasts from E18.5 Lox knockout (Lox (-/-) ) and wild type (wt) (C57BL/6) mice. Next to Lox gene depletion, mRNA expression of Lox isoforms, LOXL1-4, was significantly downregulated in Lox (-/-) bone tissue. A significant decrease of DNA synthesis of Lox (-/-) osteoblasts compared to wt was found. Early stages of osteoblastic apoptosis studied by annexin-V binding as well as later stages of DNA fragmentation were not affected. However, mineral nodule formation and osteoblastic differentiation were markedly decreased, as revealed by significant downregulation of osteoblastic markers, type I collagen, bone sialoprotein, and Runx2/Cbfa1.

引用

页码：119 / 126

页数：8

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