Paenibacillus ripae sp nov., isolated from bank side soil

A Gram-stain-variable, rod-shaped, non-motile and endospore-forming bacterium, designated strain HZ1(T), was isolated from a sample of bank side soil from Hangzhou city, Zhejiang province, PR China. On the basis of 16S rRNA gene sequence analysis, strain HZ1(T) was closely related to members of the genus Paenibacillus, sharing the highest levels of sequence similarity with Paenibacillus agarexedens DSM 1327(T) (94.4 %), Paenibacillus sputi KIT00200-70066-1(T) (94.4 %). Growth occurred at 15-42 degrees C (optimum 30-37 degrees C), pH 5.0-9.5 (optimum pH 7.0-8.0) and NaCl concentrations of up to 6.0 % (w/v) were tolerated (optimum 0.5 %). The dominant respiratory quinone was MK-7 and the DNA G+C content was 40.1 mol%. The major fatty acids were anteiso-C-15 : 0 and iso-C-16 : 0. The major polar lipids of strain HZ1(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown lipids. The diagnostic diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain HZ1(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus ripae sp. nov. (type strain HZ1(T)=CCTCC AB 2014276(T)=LMG 28639(T)) is proposed.