Role of the wbt locus of Francisella tularensis in lipopolysaccharide O-antigen biogenesis and pathogenicity

被引：81

作者：

Raynaud, Catherine

Meibom, Karin L.

Lety, Marie-Annick

Dubail, Iharilalao

Candela, Thomas

Frapy, Eric

Charbit, Alain

机构：

[1] Univ Paris Descartes, Fac Med Rene Descartes, F-75015 Paris, France

[2] INSERM, U570, Unite Pathogenie Infect Syst, F-75015 Paris, France

[3] CNRS, URA2172, Unite Toxines & Pathogenie Bacterienne, F-75724 Paris, France

来源：

INFECTION AND IMMUNITY | 2007年 / 75卷 / 01期

关键词：

D O I：

10.1128/IAI.01429-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Francisella tularensis is a highly infectious bacterial pathogen, responsible for the zoonotic disease tularemia. We screened a bank of transposon insertion mutants of F. tularensis subsp. holarctica LVS for colony morphology alterations and selected a mutant with a transposon insertion in wbtA, the first gene of the predicted lipopolysaccharide O-antigen gene cluster. Inactivation of wbtA led to the complete loss of O antigen, conferred serum sensitivity, impaired intracellular replication, and severely attenuated virulence in the mouse model. Notably, this mutant afforded protection against a challenge against virulent LVS.

引用

页码：536 / 541

页数：6

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