DETERMINATION OF METRONIDAZOLE IN ADULT ARTEMIA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

被引:7
|
作者
Cox, Sherry [1 ]
Allender, Matthew C. [2 ]
Yarbrough, Jason [1 ]
机构
[1] Univ Tennessee, Coll Vet Med, Dept Comparat Med, Knoxville, TN 37996 USA
[2] Univ Tennessee, Coll Vet Med, Dept Small Anim Clin Sci, Knoxville, TN 37996 USA
关键词
antimicrobial; aquaculture; artemia; bioencapsulation; HPLC; metronidazole; BRINE SHRIMP; HUMAN PLASMA; TROUT;
D O I
10.1080/10826070903430381
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new HPLC procedure for the determination of metronidazole, a nitroimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using methanol, samples were separated by isocratic reversed-phase HPLC on a Symmetry C18 column and quantified using UV detection at 324nm. The mobile phase was a mixture of water and methanol, with a flow-rate of 1.0ml/min. The procedure produced a linear curve over the concentration range 1-500 mu g/gm with a lower limit of quantification of 0.25 mu g/gm. Intra and inter-assay variability was less than 10%. The development of the assay allowed the determination of metronidazole in a live brine shrimp feeding study.
引用
收藏
页码:89 / 96
页数:8
相关论文
共 50 条
  • [21] Determination of meglumine in pharmaceutical formulations using high performance liquid chromatography
    Jasprica, I.
    Mrsic, N.
    Dragic, T.
    Cetina-Cizmek, B.
    PHARMAZIE, 2011, 66 (12): : 916 - 919
  • [22] Determination of Capsaicinoids in Foods Using Ultra High Performance Liquid Chromatography
    Ha, Jaeho
    Seo, Hye-Young
    Shim, You-Shin
    Seo, Dong-Won
    Seog, Homoon
    Ito, Masahito
    Nakagawa, Hiroaki
    FOOD SCIENCE AND BIOTECHNOLOGY, 2010, 19 (04) : 1005 - 1009
  • [23] Determination of firocoxib in equine plasma using high performance liquid chromatography
    Cox, S.
    Yarbrough, J.
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (02): : 205 - 208
  • [24] Determination of ceftriaxone concentrations in tissues using the high performance liquid chromatography
    Petrovic, D
    Kilibarda, V
    Jovanovic, D
    JUGOSLOVENSKA MEDICINSKA BIOHEMIJA-YUGOSLAV MEDICAL BIOCHEMISTRY, 1996, 15 (04): : 267 - 267
  • [25] Determination of capsaicinoids in foods using ultra high performance liquid chromatography
    Jaeho Ha
    Hye-Young Seo
    You-Shin Shim
    Dong-Won Seo
    Homoon Seog
    Masahito Ito
    Hiroaki Nakagawa
    Food Science and Biotechnology, 2010, 19 : 1005 - 1009
  • [27] Determination of pravastatin by high performance liquid chromatography
    Siekmeier, R
    Gross, W
    März, W
    INTERNATIONAL JOURNAL OF CLINICAL PHARMACOLOGY AND THERAPEUTICS, 2000, 38 (09) : 419 - 425
  • [28] High performance liquid chromatography determination of formitol
    Foroughifar, N
    Mobinikhaledi, A
    Tajbakhsh, M
    Naeimi, H
    ASIAN JOURNAL OF CHEMISTRY, 2002, 14 (02) : 1101 - 1103
  • [29] DETERMINATION OF METRONIDAZOLE AND ITS METABOLITES IN MOUSE-TISSUES AND BODY-FLUIDS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    CHACKO, M
    NAIR, J
    BHIDE, SV
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 1986, 23 (04): : 220 - 222
  • [30] Determination of lysinoalamine by high performance liquid chromatography
    Moret, S.
    Cherubin, S.
    Rodriguez-Estrada, M.T.
    Lercker, G.
    HRC Journal of High Resolution Chromatography, 1994, 17 (12):