DETERMINATION OF METRONIDAZOLE IN ADULT ARTEMIA USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

被引：7
|
作者：
Cox, Sherry [1 ]
Allender, Matthew C. [2 ]
Yarbrough, Jason [1 ]
机构：
[1] Univ Tennessee, Coll Vet Med, Dept Comparat Med, Knoxville, TN 37996 USA
[2] Univ Tennessee, Coll Vet Med, Dept Small Anim Clin Sci, Knoxville, TN 37996 USA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2010年 / 33卷 / 01期
关键词：
antimicrobial; aquaculture; artemia; bioencapsulation; HPLC; metronidazole; BRINE SHRIMP; HUMAN PLASMA; TROUT;
D O I：
10.1080/10826070903430381
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new HPLC procedure for the determination of metronidazole, a nitroimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using methanol, samples were separated by isocratic reversed-phase HPLC on a Symmetry C18 column and quantified using UV detection at 324nm. The mobile phase was a mixture of water and methanol, with a flow-rate of 1.0ml/min. The procedure produced a linear curve over the concentration range 1-500 mu g/gm with a lower limit of quantification of 0.25 mu g/gm. Intra and inter-assay variability was less than 10%. The development of the assay allowed the determination of metronidazole in a live brine shrimp feeding study.
引用
收藏
页码：89 / 96
页数：8
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