Quantitation of yeast ceramides using high-performance liquid chromatography-evaporative light-scattering detection

被引：18
|
作者：
Zhou, Q
Zhang, L
Fu, XQ
Chen, GQ [1 ]
机构：
[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[2] Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 780卷 / 01期
关键词：
yeast cells; ceramides;
D O I：
10.1016/S1570-0232(02)00466-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatograph equipped with an evaporative light scattering detector (ELSD) (HPLC-ELSD) was used to assay the ceramides in yeast cells. The HPLC-ELSD method employed a cyanopropyl bonded column (CN column) that effectively separated the main interfering substance ergosterol without any derivatization process; most other interfering substances were also removed. The method can be applied for routine assay of ceramide content in yeast. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码：161 / 169
页数：9
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