Isolation and characterization of microsatellite markers from Fangzheng silver crucian carp, Carassius auratus gibelio (Bloch), and cross-amplification in the closely related species crucian carp, Carassius auratus auratus (Linnaeus)

被引：5
|
作者：
Jia, Zhi-Ying
Sun, Xiao-Wen
Liang, Li-Qun
Lu, Cui-Yun
Lei, Qing-Quan
机构：
[1] Chinese Acad Fishery Sci, Heilongjiang Fisheries Res Inst, Harbin 150070, Peoples R China
[2] Shanghai Fisheries Univ, Coll Aqua Life Sci & Technol, Shanghai 200090, Peoples R China
[3] Harbin Univ Sci & Technol, Mat Sci & Engn Coll, Harbin 150080, Peoples R China
来源：
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 04期
关键词：
crucian carp; microsatellites; primers;
D O I：
10.1111/j.1471-8286.2006.01464.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the development of 20 microsatellite markers for Fangzheng silver crucian carp, Carassius auratus gibelio (Bloch). Nineteen out of 20 showed polymorphism with alleles ranging from two to 14. These loci were screened to amplify the closely related species crucian carp, Carassius auratus auratus (Linnaeus) and all of them can amplify DNA with the size similar to the former. The origin of silver crucian carp is in issue and the population genetic structure is still unclear. Microsatellite markers isolated from the silver crucian carp and their utility in the crucian carp will be useful for these researches.
引用
收藏
页码：1141 / 1143
页数：3
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