Atomic force microscopic study of folate receptors in live cells with functionalized tips

被引：14
|
作者：
Girish, C. M. [1 ]
Binulal, N. S. [1 ]
Anitha, V. C. [1 ]
Nair, Shantikumar [1 ]
Mony, U. [1 ]
Prasanth, R. [1 ]
机构：
[1] Amrita Viswavidyapeetham Univ, Amrita Ctr Nanosci, Cochin 682041, Kerala, India
来源：
APPLIED PHYSICS LETTERS | 2009年 / 95卷 / 22期
关键词：
atomic force microscopy; biomedical imaging; biomembranes; cancer; cellular biophysics; molecular biophysics; tumours; RECOGNITION EVENTS; AFM; LOCALIZATION; ANTIBODIES;
D O I：
10.1063/1.3262966
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
Membrane associated folate receptors (FR) is gaining importance in cancer research. Understanding the FR density, distribution, and the strength of its interaction with ligands is crucial in cancer diagnostics and therapeutics. Here we reported the enhanced phase contrast image of FR by scanning with properly functionalized atomic force microscope (AFM) tips over live cell lines. The choice of the ligand was made for better interaction of tip with FR, expressed in the cell lines. The selectively enhanced force of interaction at the receptor molecule produced a considerable enhancement in the phase contrast between a receptor site and a nonreceptor site.
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页数：3
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