VALIDATION OF METHODOLOGY FOR THE CHEMICAL CHARACTERIZATION OF SUGAR CANE BAGASSE.

被引：215

作者：

Gouveia, Ester Ribeiro ^{[1
]}

do Nascimento, Renata Trajano ^{[1
]}

Souto-Maior, Ana Maria ^{[1
]}

de Moraes Rocha, George Jackson ^{[2
]}

机构：

[1] Univ Fed Pernambuco, Dept Antibiot, BR-50670901 Recife, PE, Brazil

[2] Univ Sao Paulo, Escola Engn Lorena, BR-12602810 Lorena, SP, Brazil

来源：

QUIMICA NOVA | 2009年 / 32卷 / 06期

关键词：

bagasse; HPLC; validation;

D O I：

10.1590/S0100-40422009000600026

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

VALIDATION OF METHODOLOGY FOR THE CHEMICAL CHARACTERIZATION OF SUGAR CANE BAGASSE. In this work, a methodology for the characterization of sugar cane bagasse was validated. Bagasse pre-treated with steam in a 5000 L reactor at a pressure of 15.3 kgf/cm(2), during 7 min, was used to test the methodology. The methodology consisted of the hydrolysis of the material with H(2)SO(4) at 72% v/v, for the quantification of carbohydrates, organic acid, furfural and hydroxymethylfurfural by HPLC; insoluble lignin and ash by gravimetry; and soluble lignin by spectrophotometry. Linearity, repeatability, reproducibility and accuracy of the results obtained in two Research Laboratories were determined, and were considered to be suitable for the validation of the methodology.

引用

页码：1500 / 1503

页数：4

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