Visualization of intracellular CA2+ dynamics with simultaneous 2-photon excited fluorescence and third harmonic generation microscope

被引：1
|
作者：
Rivet, S
Canioni, L
Sarger, L
Barille, R
Vacher, P
Ducret, T
机构：
[1] CNRS, UMR 6136, Lab Proprietes Opt Mat & Applicat, F-49016 Angers, France
[2] CNRS, UMR 5798, Ctr Phys Mol & Ondes Hertziennes, F-33405 Talence, France
[3] CNRS, UMR 5543, Lab Physiol& Physiopathol Signalisat Cellulaire, F-33076 Bordeaux, France
来源：
JOURNAL OF FLUORESCENCE | 2002年 / 12卷 / 02期
关键词：
intracellular CA(2+) dynamics; 2-photon excited fluorescence; third harmonic generation microscopy;
D O I：
10.1023/A:1016804615939
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We show that the simultaneous coupling of two laser scanning non-linear microscopies (2-photon excited fluorescence and third harmonic generation microscopy) give functional and morphological information for improving our understanding on intracellular calcium flows.
引用
收藏
页码：197 / 199
页数：3
相关论文
共 50 条
  • [31] 2-PHOTON RESONANT 3RD-HARMONIC GENERATION IN POLYSILANES
    HASEGAWA, T
    IWASA, Y
    KISHIDA, H
    KODA, T
    TOKURA, Y
    TACHIBANA, H
    KAWABATA, Y
    PHYSICAL REVIEW B, 1992, 45 (11): : 6317 - 6320
  • [32] SIMULTANEOUS MEASUREMENT OF INTRACELLULAR PH AND CA2+ USING THE FLUORESCENCE OF SNARF-1 AND FURA-2
    MARTINEZZAGUILAN, R
    MARTINEZ, GM
    LATTANZIO, F
    GILLIES, RJ
    AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (02): : C297 - C307
  • [33] EFFECT OF 2-PHOTON RADIATION ABSORPTION ON THE 2ND-HARMONIC GENERATION IN CRYSTALS
    DMITRIEV, VG
    KONOVALOV, VA
    KVANTOVAYA ELEKTRONIKA, 1979, 6 (03): : 500 - 505
  • [34] PECULIARITIES OF THE 2-PHOTON EXCITED COUMARIN DYE SOLUTION FLUORESCENCE-SPECTRA
    SMIRNOVA, TN
    TIKHONOV, EA
    SHPAK, MT
    UKRAINSKII FIZICHESKII ZHURNAL, 1981, 26 (09): : 1448 - 1455
  • [35] POLARIZATION OF 2-PHOTON EXCITED FLUORESCENCE OF COMPLEX ORGANIC-MOLECULES IN VAPORS
    BLOKHIN, AP
    POVEDAILO, VA
    TOLKACHEV, VA
    OPTIKA I SPEKTROSKOPIYA, 1986, 60 (01): : 60 - 64
  • [36] Regulation of airway ciliary activity by Ca2+:: Simultaneous measurement of beat frequency and intracellular Ca2+
    Lansley, AB
    Sanderson, MJ
    BIOPHYSICAL JOURNAL, 1999, 77 (01) : 629 - 638
  • [37] High-resolution simultaneous three-photon fluorescence and third-Harmonic-generation microscopy
    Chu, SW
    Tai, SP
    Ho, CL
    Lin, CH
    Sun, CK
    MICROSCOPY RESEARCH AND TECHNIQUE, 2005, 66 (04) : 193 - 197
  • [38] LASER 2-PHOTON EXCITED FLUORESCENCE DETECTOR FOR MICROBORE LIQUID-CHROMATOGRAPHY
    PFEFFER, WD
    YEUNG, ES
    ANALYTICAL CHEMISTRY, 1986, 58 (09) : 2103 - 2105
  • [39] SIMULTANEOUS 2-PHOTON ABSORPTION AND SUM-FREQUENCY GENERATION IN ZNO
    COLLES, MJ
    HOLAH, GD
    BULLETIN OF THE AMERICAN PHYSICAL SOCIETY, 1974, 19 (03): : 308 - 308
  • [40] Frequency of elemental events of intracellular Ca2+ dynamics
    Thul, R.
    Falcke, M.
    PHYSICAL REVIEW E, 2006, 73 (06):
12345