Switch of cadherin expression from E- to N-type during the activation of rat hepatic stellate cells

被引:42
|
作者
Lim, Young-Suk
Lee, Han Chu
Lee, Hyo-Suk
机构
[1] Seoul Natl Univ, Coll Med, Dept Internal Med, Seoul 110744, South Korea
[2] Seoul Natl Univ, Coll Med, Liver Res Inst, Seoul 110744, South Korea
[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Gastroenterol, Seoul 138736, South Korea
关键词
E-cadherin; N-cadherin; beta-catenin; hepatic stellate cells;
D O I
10.1007/s00418-006-0233-y
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The activation of hepatic stellate cell (HSC) is a common pathway leading to hepatic fibrosis. However, the molecular mechanisms underlying HSC activation remain obscure. To elucidate the nature of the HSC activation, we investigated the expression of E-cadherin and its switch to N-cadherin during rat HSC activation, in vivo and in vitro. Immunohistochemical and immunocytochemical staining were performed to identify the expressions of E-cadherin, N-cadherin, and beta-catenin in rat HSCs, in vivo and in vitro. Serial changes in the expressions of these adhesion molecules during the spontaneous activation of cultured rat HSCs were also demonstrated by RT-PCR and by immunoblotting. E-cadherin and beta-catenin were expressed on opposing cell membranes of GFAP-positive rat HSCs and adjacent hepatocytes in vivo, and between desmin-positive rat HSCs in vitro. With the progression of rat HSC activation in tissue and in culture, E-cadherin disappeared gradually, whereas N-cadherin appeared at the cell periphery. The results of RT-PCR and immunoblotting were concordant with immunocytochemistry findings. In conclusion, resting rat HSCs express E-cadherin and beta-catenin both in vivo and in vitro, and E-cadherin switches to N-cadherin during HSC activation. These results suggest that HSC activation represents transdifferentiation from an epithelial to a mesenchymal phenotype.
引用
收藏
页码:149 / 160
页数:12
相关论文
共 50 条
  • [31] Cadherin switch from E- to N-cadherin in melanoma progression is regulated by the PI3K/PTEN pathway through Twist and Snail
    Hao, L.
    Ha, J. R.
    Kuzel, P.
    Garcia, E.
    Persad, S.
    BRITISH JOURNAL OF DERMATOLOGY, 2012, 166 (06) : 1184 - 1197
  • [32] Role of the Ets-1 transcription factor during activation of rat hepatic stellate cells in culture
    Knittel, T
    Kobold, D
    Dudas, J
    Saile, B
    Ramadori, G
    AMERICAN JOURNAL OF PATHOLOGY, 1999, 155 (06): : 1841 - 1848
  • [33] Expression of alpha(5) and beta(1) integrin subunits during activation of hepatic stellate cells.
    McMorrow, BC
    Milliano, MT
    ONeill, R
    Luxon, BA
    GASTROENTEROLOGY, 1997, 112 (04) : A1331 - A1331
  • [34] Gene expression and regulation of plasminogen activator inhibitor type I in hepatic stellate cells of rat liver
    Knittel, T
    Fellmer, P
    Ramadori, G
    GASTROENTEROLOGY, 1996, 111 (03) : 745 - 754
  • [35] Expression of retinoic acid receptor beta protein is increased during activation of hepatic stellate cells.
    Jones, EH
    Benyon, RC
    HEPATOLOGY, 1998, 28 (04) : 186A - 186A
  • [36] A SWITCH FROM E-CADHERIN TO N-CADHERIN EXPRESSION AS A NOVEL PREDICTOR FOR INTRAVESICAL RECURRENCE OF SUPERFICIAL BLADDER CANCER
    Muramaki, Mototsugu
    Miyake, Hideaki
    Takenaka, Atsushi
    Fujisawa, Masato
    JOURNAL OF UROLOGY, 2009, 181 (04): : 637 - 637
  • [37] Ovarian N- and E-cadherin expression during the rat estrous cycle.
    Machell, NH
    Blaschuk, OW
    Farookhi, R
    BIOLOGY OF REPRODUCTION, 1998, 58 : 106 - 106
  • [38] Up-regulation of uPARAP/Endo180 during culture activation of rat hepatic stellate cells and its presence in hepatic stellate cell lines from different species
    Seyed A Mousavi
    Marita S Fønhus
    Trond Berg
    BMC Cell Biology, 10
  • [39] Isolation of a stellate cell activation-associated protein (STAP) with peroxidase activity from rat hepatic stellate cells.
    Kawada, N
    Kristensen, DB
    Asahina, K
    Yoshizato, K
    HEPATOLOGY, 2001, 34 (04) : 401A - 401A
  • [40] Up-regulation of uPARAP/Endo180 during culture activation of rat hepatic stellate cells and its presence in hepatic stellate cell lines from different species
    Mousavi, Seyed A.
    Fonhus, Marita S.
    Berg, Trond
    BMC CELL BIOLOGY, 2009, 10