Gene expression of a class I chitinase (Vcchit.1b) in the skin of table grapes was analyzed as a molecular marker for changes induced at low-temperature and also to study the effect of high CO2 levels modulating transcript levels at 0 degrees C. An active recombinant VcCHIT1b was overexpressed in Escherichia coli, and as the protein was produced as insoluble inclusion bodies, it was solubilized and refolded. The purified recombinant chitinase showed an optimum pH of 6.0 and a temperature of 50 degrees C, retaining activity at 0 and -10 degrees C. Purified chitinase exerted in vitro antifungal activity against Botrytis cinerea. Furthermore, recombinant chitinase was able to cryoprotect lactate dehydrogenase against freeze/thaw inactivation. However, the recombinant VcCHIT1b did not show any antifreeze activity when the thermal hysteresis activity was measured using differential scanning calorimetry.
机构:
Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Cooperat Res Ctr Viticulture, Glen Osmond, SA 5064, Australia
Univ Stellenbosch, Dept Viticulture & Oenol, ZA-7602 Stellenbosch, South AfricaUniv Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Bindon, K.
Dry, P.
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Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Cooperat Res Ctr Viticulture, Glen Osmond, SA 5064, AustraliaUniv Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Dry, P.
Loveys, B.
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Cooperat Res Ctr Viticulture, Glen Osmond, SA 5064, Australia
CSIRO, Plant Ind Hort Unit, Glen Osmond, SA 5064, AustraliaUniv Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
机构:
Univ Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, GermanyUniv Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany
Kolb, CA
Wirth, E
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Univ Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, GermanyUniv Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany
Wirth, E
Kaiser, WM
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Univ Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, GermanyUniv Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany
Kaiser, WM
Meister, A
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Univ Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, GermanyUniv Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany
Meister, A
Riederer, M
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Univ Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, GermanyUniv Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany
Riederer, M
Pfündel, EE
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Univ Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, GermanyUniv Wurzburg, Julius Von Sachs Inst Biowissensch, D-97082 Wurzburg, Germany
机构:
Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Australian Wine Res Inst, Adelaide, SA 5064, AustraliaUniv Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Kneknopoulos, Petros
Skouroumounis, George K.
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Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, AustraliaUniv Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Skouroumounis, George K.
Hayasaka, Yoji
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Australian Wine Res Inst, Adelaide, SA 5064, AustraliaUniv Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia
Hayasaka, Yoji
Taylor, Dennis K.
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Univ Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, AustraliaUniv Adelaide, Sch Agr Food & Wine, Glen Osmond, SA 5064, Australia