MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2

被引:19
|
作者
Wang Haiyan [1 ]
Zhao Fangfang [2 ]
Cai Shanbao [3 ]
Pu Youguang [2 ]
机构
[1] Univ Sci & Technol China, Dept Clin Geriatr, Div Life Sci & Med, Affiliated Hosp 1, Hefei 230001, Anhui, Peoples R China
[2] Univ Sci & Technol China, Dept Lab Res Ctr, Anhui Prov Canc Hosp, Div Life Sci & Med,West Branch,Affiliated Hosp 1, Hefei 230001, Anhui, Peoples R China
[3] Univ Sci & Technol China, Dept Orthoped Surg, Anhui Prov Canc Hosp, Div Life Sci & Med,West Branch,Affiliated Hosp 1, Hefei 230001, Anhui, Peoples R China
来源
JOURNAL OF BONE ONCOLOGY | 2019年 / 17卷
关键词
Osteosarcoma; Chemoresistance; DNA methylation; miR-193a-3p; IRS2; MIRNA EXPRESSION; DNA METHYLATION; MICRORNA; RESISTANCE; SUPPRESS; MIR-9-3; DRUG;
D O I
10.1016/j.jbo.2019.100241
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Chemoresistance prevents curative potential of chemotherapy in most cases. MicroRNAs (miRNAs) are key players in regulating chemoresistance in osteosarcoma, which is the most common primary bone cancer. Bisulfite sequencing and quantitative real time PCR analyses showed that miR-193a expression is downregulated by DNA hypermethylation at its promoter region in a chemoresistant cell line, SJSA-1, compared to a chemosensitive cell line G-292. Introduction of a miR-193a mimic in SJSA-1 cells or an antagomir into G-292 cells confirmed the role of miR-193a in osteosarcoma chemoresistance. Bioinformatics together with biochemical assays showed that insulin receptor substrate 2 (IRS2) is a target of miR-193a. Our data concludes that miR-193a plays a role in the osteosarcoma chemoresistance and thus might serve as a useful biomarker for osteosarcoma prognosis.
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收藏
页数:9
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