siRNA-directed inhibition of HIV-1 infection

被引：626
|
作者：
Novina, CD [1 ]
Murray, MF
Dykxhoorn, DM
Beresford, PJ
Riess, J
Lee, SK
Collman, RG
Lieberman, J
Shankar, P
Sharp, PA
机构：
[1] Harvard Univ, Sch Med, Ctr Blood Res, Boston, MA 02115 USA
[2] MIT, Ctr Canc Res, Cambridge, MA 02139 USA
[3] MIT, Dept Biol, Cambridge, MA USA
[4] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
[5] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA
[6] Univ Penn, Sch Med, Philadelphia, PA 19104 USA
来源：
NATURE MEDICINE | 2002年 / 8卷 / 07期
关键词：
D O I：
10.1038/nm725
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.
引用
收藏
页码：681 / 686
页数：6
相关论文
共 50 条
  • [31] Potential treatment of HIV infection using miRNA-siRNA hybrids to inhibit HIV-1 replication
    Yam, P.
    Lo, H.
    Marcovecchio, P. M.
    Chang, T.
    Zaia, J. A.
    Yee, J.
    TRANSFUSION, 2007, 47 (03) : 209A - 209A
  • [32] Combinatorial siRNA constructs for HIV-1 therapy
    Anderson, J
    Banerjea, A
    Rossi, J
    Akkina, R
    MOLECULAR THERAPY, 2003, 7 (05) : S265 - S265
  • [33] Mixed cryoglobulinemia in HIV-1 infection: The role of HIV-1
    Dimitrakopoulos, AN
    Kordossis, T
    Hatzakis, A
    Moutsopoulos, HM
    ANNALS OF INTERNAL MEDICINE, 1999, 130 (03) : 226 - 230
  • [34] A case of HIV-1 superinfection in chronic HIV-1 infection
    Longwill, S.
    Hourihan, M.
    Apea, V.
    HIV MEDICINE, 2010, 11 : 36 - 36
  • [35] Aptamer-siRNA Conjugate Directed Transcriptional Gene Silencing in HIV-1 Infected T Cells
    Lazar, Daniel C.
    Zhou, Jiehau
    Saayman, Sheena
    Rossi, John J.
    Morris, Kevin V.
    Weinburg, Marc S.
    MOLECULAR THERAPY, 2015, 23 : S27 - S28
  • [36] Receptor-targeted aptamer-siRNA conjugate-directed transcriptional regulation of HIV-1
    Zhou, Jiehua
    Lazar, Daniel
    Li, Haitang
    Xia, Xin
    Satheesan, Sangeetha
    Charlins, Paige
    O'Mealy, Denis
    Akkina, Ramesh
    Saayman, Sheena
    Weinberg, Marc S.
    Rossi, John J.
    Morris, Kevin V.
    THERANOSTICS, 2018, 8 (06): : 1575 - 1590
  • [37] Effective inhibition of HIV-1 isolated from patients with acute primary HIV-1 infection by aminooxypentane-RANTES
    Rusconi, S
    Merrill, DP
    La Seta-Catamancio, S
    Citterio, P
    Offord, RE
    Hirsch, MS
    AIDS, 1999, 13 (09) : 1144 - 1145
  • [38] How Can Plant DNA Viruses Evade siRNA-Directed DNA Methylation and Silencing?
    Pooggin, Mikhail M.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (08): : 15233 - 15259
  • [39] Molecular signatures of T-cell inhibition in HIV-1 infection
    Larsson, Marie
    Shankar, Esaki M.
    Che, Karlhans F.
    Saeidi, Alireza
    Ellegard, Rada
    Barathan, Muttiah
    Velu, Vijayakumar
    Kamarulzaman, Adeeba
    RETROVIROLOGY, 2013, 10
  • [40] Thermodynamic-based approach to the inhibition of HIV-1 cell infection
    Freire, E.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2015, 249
12345