siRNA-directed inhibition of HIV-1 infection

被引：626
|
作者：
Novina, CD [1 ]
Murray, MF
Dykxhoorn, DM
Beresford, PJ
Riess, J
Lee, SK
Collman, RG
Lieberman, J
Shankar, P
Sharp, PA
机构：
[1] Harvard Univ, Sch Med, Ctr Blood Res, Boston, MA 02115 USA
[2] MIT, Ctr Canc Res, Cambridge, MA 02139 USA
[3] MIT, Dept Biol, Cambridge, MA USA
[4] MIT, McGovern Inst Brain Res, Cambridge, MA 02139 USA
[5] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA
[6] Univ Penn, Sch Med, Philadelphia, PA 19104 USA
来源：
NATURE MEDICINE | 2002年 / 8卷 / 07期
关键词：
D O I：
10.1038/nm725
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.
引用
收藏
页码：681 / 686
页数：6
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