Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris

被引：32
|
作者：
Ecamilla-Treviño, LL [1 ]
Viader-Salvadó, JM [1 ]
Barrera-Saldaña, HA [1 ]
Guerrero-Olazarán, M [1 ]
机构：
[1] Univ Autonoma Nuevo Leon, Fac Med, Dept Bioquim, Monterrey 64460, NL, Mexico
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 02期
关键词：
alcohol oxidase; gene expression; human growth hormone; Pichia pastoris;
D O I：
10.1023/A:1005675920451
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Mature human growth hormone (hGH) cDNA was cloned by homologous recombination into the yeast Pichia pastoris genome. The hGH gene expression was placed under the control of the methanol-inducible alcohol oxidase 1 (AOX1) gene promoter and the Saccharomyces cerevisiae alpha-factor signal sequence to direct the secretion of recombinant human growth hormone (rhGH) into the growth medium. O-2-limited induction of recombinant yeast strains in shake tubes with 3 ml of culture medium produced up to 11 mg rhGH l(-1), while high cell density cultures using a 2-l bioreactor produced about 49 mg rhGH l(-1) achieving 40% of total protein of the culture medium supernatant.
引用
收藏
页码：109 / 114
页数：6
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