Metabolite Profile of Cervicovaginal Fluids from Early Pregnancy Is Not Predictive of Spontaneous Preterm Birth

被引:15
|
作者
Thomas, Melinda M. [1 ]
Sulek, Karolina [1 ]
McKenzie, Elizabeth J. [1 ]
Jones, Beatrix [2 ]
Han, Ting-Li [1 ]
Villas-Boas, Silas G. [3 ]
Kenny, Louise C. [4 ]
McCowan, Lesley M. E. [5 ]
Baker, Philip N. [1 ]
机构
[1] Univ Auckland, Liggins Inst, Auckland 1023, New Zealand
[2] Massey Univ, Inst Nat & Math Sci, Albany 0632, New Zealand
[3] Univ Auckland, Sch Biol Sci, Auckland 1010, New Zealand
[4] Natl Univ Ireland Univ Coll Cork, Irish Ctr Fetal & Neonatal Translat Res, Cork, Ireland
[5] Univ Auckland, Dept Obstet & Gynaecol, Auckland 1023, New Zealand
基金
爱尔兰科学基金会;
关键词
metabolomics; cervicovaginal; biomarkers; spontaneous preterm birth; MATERNAL SERUM; RISK-FACTORS; METABOLOMICS; BIOMARKERS; DISCOVERY; PROTEIN; TIME;
D O I
10.3390/ijms161126052
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In our study, we used a mass spectrometry-based metabolomic approach to search for biomarkers that may act as early indicators of spontaneous preterm birth (sPTB). Samples were selected as a nested case-control study from the Screening for Pregnancy Endpoints (SCOPE) biobank in Auckland, New Zealand. Cervicovaginal swabs were collected at 20 weeks from women who were originally assessed as being at low risk of sPTB. Samples were analysed using gas chromatography-mass spectrometry (GC-MS). Despite the low amount of biomass (16-23 mg), 112 compounds were detected. Statistical analysis showed no significant correlations with sPTB. Comparison of reported infection and plasma inflammatory markers from early pregnancy showed two inflammatory markers were correlated with reported infection, but no correlation with any compounds in the metabolite profile was observed. We hypothesise that the lack of biomarkers of sPTB in the cervicovaginal fluid metabolome is simply because it lacks such markers in early pregnancy. We propose alternative biofluids be investigated for markers of sPTB. Our results lead us to call for greater scrutiny of previously published metabolomic data relating to biomarkers of sPTB in cervicovaginal fluids, as the use of small, high risk, or late pregnancy cohorts may identify metabolite biomarkers that are irrelevant for predicting risk in normal populations.
引用
收藏
页码:27741 / 27748
页数:8
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