Isolation and characterization of polymorphic microsatellites for assessment of genetic variation of hops (Humulus lupulus L.)

被引：28

作者：

Hadonou, AM ^{[1
]}

Walden, R

Darby, P

Darby, W

机构：

[1] Hort Res Int, Maidstone ME19 6BJ, Kent, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Hop Res, Hort Res Int, Ashford TN25 5AH, Kent, England

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 02期

关键词：

genetic variation; hop; Humulus lupulus (L.); microsatellite;

D O I：

10.1111/j.1471-8286.2004.00641.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hop (Humulus lupulus L.) cultivars are vegetatively propagated and it is difficult to differentiate them during the process of propagation. Fingerprinting with molecular markers based on DNA could be a useful means of identifying different cultivars. Simple sequence repeats, or microsatellite markers, are the most suitable marker for genetic fingerprinting because they are multi-allelic and co-dominant. For this purpose, we have developed primers for 10 new polymorphic microsatellite loci that are suitable for genetic fingerprinting in hop.

引用

页码：280 / 282

页数：3

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