Isolation and characterization of polymorphic microsatellites for assessment of genetic variation of hops (Humulus lupulus L.)

被引：28
|
作者：
Hadonou, AM [1 ]
Walden, R
Darby, P
Darby, W
机构：
[1] Hort Res Int, Maidstone ME19 6BJ, Kent, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Hop Res, Hort Res Int, Ashford TN25 5AH, Kent, England
来源：
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 02期
关键词：
genetic variation; hop; Humulus lupulus (L.); microsatellite;
D O I：
10.1111/j.1471-8286.2004.00641.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hop (Humulus lupulus L.) cultivars are vegetatively propagated and it is difficult to differentiate them during the process of propagation. Fingerprinting with molecular markers based on DNA could be a useful means of identifying different cultivars. Simple sequence repeats, or microsatellite markers, are the most suitable marker for genetic fingerprinting because they are multi-allelic and co-dominant. For this purpose, we have developed primers for 10 new polymorphic microsatellite loci that are suitable for genetic fingerprinting in hop.
引用
收藏
页码：280 / 282
页数：3
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