N-linked oligosaccharide structures in the diamine oxidase from porcine kidney

被引：0
|
作者：
Huang, YP [1 ]
Mechref, Y [1 ]
Novotny, MV [1 ]
机构：
[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA
来源：
CARBOHYDRATE RESEARCH | 2000年 / 323卷 / 1-4期
关键词：
exoglycosidases; N-glycan; HPAEC; MALDI/TOF-MS; porcine kidney diamine oxidase;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The oligosaccharide sequences present in DAO were conclusively determined using specific exoglycosidases in conjunction with MALDI/TOF-MS. The structures found in the,glycoprotein are primarily linear, di-, or tribranched fucosylated complex type. RIS analysis of the esterified N-glycan pool derived from DAO indicated the presence of several di- and trisialylated structures. (C) 2000 Elsevier Science Ltd. All rights reserved.
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页码：111 / 125
页数：15
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