Graphene oxide induces autophagy and apoptosis via ROS-dependent AMPK/mTOR/ULK-1 pathway in colorectal cancer cells

被引：19
|
作者：
Shen, Jiamen [1 ]
Dong, Jiatian [1 ]
Shao, Feng [2 ]
Zhao, Jiaying [1 ]
Gong, Lifeng [1 ]
Wang, Huipeng [1 ]
Chen, Wenjie [1 ]
Zhang, Yafei [2 ]
Cai, Yuankun [1 ]
机构：
[1] Fudan Univ, Peoples Hosp Shanghai 5, Dept Gen Surg, Shanghai, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Minist Educ, Key Lab Thin Film & Microfabricat Technol, Shanghai, Peoples R China
来源：
NANOMEDICINE | 2022年 / 17卷 / 09期
关键词：
apoptosis; autophagy; colorectal cancer; graphene oxide; reactive oxygen species; OXIDATIVE STRESS; DNA-DAMAGE; CYTOTOXICITY; NANOCOMPOSITE; NANOPARTICLES;
D O I：
10.2217/nnm-2022-0030
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: To investigate the anticancer effects and action mechanism of graphene oxide (GO) in colorectal cancer (CRC). Materials & methods: Anticancer effects and mechanisms of GO in CRC were investigated both in vivo and in vitro. Results: GO significantly inhibited tumor growth both in vitro and in vivo. GO was able to enter HCT116 cells through endocytosis. GO treatment resulted in cytotoxicity, reactive oxygen species (ROS) production, apoptosis, autophagy and activation of the AMPK/mTOR/ULK1 signal pathway. However, ROS scavenger N-acetylcysteine (NAC) attenuated the above effects and restored the effects of GO on protein expressions related to apoptosis, autophagy and AMPK/mTOR/ULK1 signal pathways. Conclusion: GO exerts anticancer effects against CRC via ROS-dependent AMPK/mTOR/ULK-1 pathway-related autophagy and apoptosis.
引用
收藏
页码：591 / 605
页数：15
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