A Novel Recombinant Chlorophyllase1 from Chlamydomonas reinhardtii for the Production of Chlorophyllide Derivatives

被引：15
|
作者：
Chou, Yi-Li [1 ]
Ko, Chia-Yun [2 ]
Yen, Chih-Chung [3 ,4 ]
Chen, Long-Fang O. [2 ]
Shaw, Jei-Fu [1 ,4 ]
机构：
[1] I Shou Univ, Dept Biol Sci & Technol, Kaohsiung 82445, Taiwan
[2] Acad Sinica, Inst Plant & Microbial Biol, Taipei 11529, Taiwan
[3] Natl Chung Hsing Univ, Inst Genom & Bioinformat, Taichung 40227, Taiwan
[4] Natl Chung Hsing Univ, Agr Biotechnol Ctr, Taichung 40227, Taiwan
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2015年 / 63卷 / 43期
关键词：
Chlamydomonas reinhardtii; chlorophyllase; chlorophyllide derivatives; enzyme kinetics; LEAF SENESCENCE; CHLORELLA-PROTOTHECOIDES; DEGRADATION; BREAKDOWN; PURIFICATION; HYDROLASE; ENZYME; PHEOPHORBIDE; BIOSYNTHESIS; CLONING;
D O I：
10.1021/acs.jafc.5b02787
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Natural chlorophyll metabolites have exhibited physiological activity in vitro. In this study, a recombinant chlorophyllase1 gene from Chlamydomonas reinhardtii (CrCLH1) was isolated and characterized. Recombinant CrCLH1 can perform chlorophyll dephytylation and produce chlorophyllide and phytol. In a transient assay, the subcellular localization of CrCLH1-green fluorescent protein was determined to be outside the chloroplast. Biochemical analyses of the activity of recombinant CrCLH1 indicated that its optimal pH value and temperature are 6.0 and 40 degrees C, respectively. Enzyme kinetic data revealed that the recombinant CrCLH1 had a higher catalytic efficiency for chlorophyll a than for chlorophyll b and bacteriochlorophyll a. According to high-performance liquid chromatography analysis of chlorophyll hydrolysis, recombinant CrCLH1 catalyzed the conversion of chlorophyll a to pheophorbide a at pH 5. Therefore, recombinant CrCLH1 can be used as a biocatalyst to produce chlorophyllide derivatives.
引用
收藏
页码：9496 / 9503
页数：8
相关论文
共 50 条
  • [21] Nuclear genome shuffling significantly increases production of chloroplast-based recombinant protein in Chlamydomonas reinhardtii
    Fields, Francis J.
    Ostrand, Joseph T.
    Tran, Miller
    Mayfield, Stephen P.
    ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2019, 41
  • [22] High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii
    Freudenberg, Robert A.
    Baier, Thomas
    Einhaus, Alexander
    Wobbe, Lutz
    Kruse, Olaf
    BIORESOURCE TECHNOLOGY, 2021, 323
  • [23] Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome
    McQuillan, Josie L.
    Berndt, Anthony J.
    Sproles, Ashley E.
    Mayfield, Stephen P.
    Pandhal, Jagroop
    NEW BIOTECHNOLOGY, 2022, 68 : 9 - 18
  • [24] Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome
    McQuillan, Josie L.
    Berndt, Anthony J.
    Sproles, Ashley E.
    Mayfield, Stephen P.
    Pandhal, Jagroop
    New Biotechnology, 2022, 68 : 9 - 18
  • [25] Optimized production of a bioactive human recombinant protein from the microalgae Chlamydomonas reinhardtii grown at high density in a fed-batch bioreactor
    Torres-Tiji, Yasin
    Fields, Francis J.
    Yang, Yanping
    Heredia, Vanessa
    Horn, Svein Jarle
    Keremane, Saumya R.
    Jin, Moonsoo M.
    Mayfield, Stephen P.
    ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2022, 66
  • [26] FUNCTIONAL CHARACTERIZATION OF THE NOVEL GENE CIA7 FROM CHLAMYDOMONAS REINHARDTII
    Cantu, Hector Guillermo Gonzalez
    Ynalvez, Ruby Agno
    FASEB JOURNAL, 2018, 32 (01):
  • [27] Filaments and fingers: Novel structural aspects of the single septin from Chlamydomonas reinhardtii
    Pinto, Andressa P. A.
    Pereira, Humberto M.
    Zeraik, Ana E.
    Ciol, Heloisa
    Ferreira, Frederico M.
    Brandao-Neto, Jose
    DeMarco, Ricardo
    Navarro, Marcos V. A. S.
    Risi, Cristina
    Galkin, Vitold E.
    Garratt, Richard C.
    Araujo, Ana P. U.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 292 (26) : 10899 - 10911
  • [28] Borrowing genes from Chlamydomonas reinhardtii for free fatty acid production in engineered cyanobacteria
    Ruffing, Anne M.
    JOURNAL OF APPLIED PHYCOLOGY, 2013, 25 (05) : 1495 - 1507
  • [29] Borrowing genes from Chlamydomonas reinhardtii for free fatty acid production in engineered cyanobacteria
    Anne M. Ruffing
    Journal of Applied Phycology, 2013, 25 : 1495 - 1507
  • [30] Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii
    Lauersen, Kyle J.
    Wichmann, Julian
    Baier, Thomas
    Kampranis, Sotirios C.
    Pateraki, Irini
    Moller, Birger Lindberg
    Kruse, Olaf
    METABOLIC ENGINEERING, 2018, 49 : 116 - 127
12345