Isolation and characterization of a new trypsin inhibitor from Crotalaria paulina seeds

被引：16
|
作者：
Pando, LA
Di Ciero, L
Novello, JC
Oliveira, B
Weder, JKP
Marangoni, S [1 ]
机构：
[1] Univ Estadual Campinas, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP, Brazil
[2] Univ Sao Paulo, ESALQ, Dept Fitopatol, Piracicaba, SP, Brazil
[3] Tech Univ Munich, Inst Lebensmittelchem, D-85748 Garching, Germany
来源：
IUBMB LIFE | 1999年 / 48卷 / 05期
关键词：
Crotalaria paulina; trypsin inhibitor;
D O I：
10.1080/152165499306621
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new trypsin inhibitor (CPTI) has been isolated from Crotalaria paulina seeds. Purification of the inhibitor was carried out by gel filtration, ion-exchange chromatography, and subsequent reversed-phase HPLC. The presence of a single polypeptide chain, with a molecular mass of 20 kDa and isoelectric point 4.0, was detected. The trypsin inhibitor had a K-i value of 4.5 x 10(-8) M and was capable of acting on human, bovine, and porcine trypsin and weakly on bovine chymotrypsin, Amino acid analysis showed that CPTI has a high content of aspartate, glutamate, leucine, serine, and glycine, having 177 amino acid residues in its composition. These data suggest that the protein belongs to the Kunitz-type trypsin inhibitors.
引用
收藏
页码：519 / 523
页数：5
相关论文
共 50 条
  • [31] ISOLATION AND PARTIAL CHARACTERIZATION OF A TRYPSIN-INHIBITOR FROM WHEAT ENDOSPERM
    MITSUNAGA, T
    KIMURA, Y
    SHIMIZU, M
    JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 1982, 28 (04) : 419 - 429
  • [32] Isolation and characterization of a novel trypsin inhibitor from fresh lily bulbs
    Zhang, Xiaoqing
    Wang, Hexiang
    Ng, Tzi Bun
    PLANTA MEDICA, 2008, 74 (05) : 546 - 550
  • [33] ISOLATION AND CHARACTERIZATION OF URINARY TRYPSIN INHIBITOR FROM HUMAN PREGNANCY URINE
    PROKSCH, GJ
    ABADI, DM
    ROUTH, JI
    CLINICAL CHEMISTRY, 1967, 13 (08) : 680 - &
  • [34] ISOLATION AND PARTIAL CHARACTERIZATION OF A TRYPSIN-INHIBITOR FROM WHEAT BRAN
    MITSUNAGA, T
    SHIMIZU, M
    JOURNAL OF THE AGRICULTURAL CHEMICAL SOCIETY OF JAPAN, 1982, 56 (01): : 7 - 12
  • [35] ISOLATION AND CHARACTERIZATION OF PANCREATIC LIPASE INHIBITOR FROM RAPESEED SEEDS
    Krusir, Galyna
    Pylypenko, Liudmyla
    Sevastyanova, Elena
    Mazurenko, Kseniia
    Moshtakov, Serhii
    Shunko, Hanna
    Vitiuk, Antonina
    Shpyrko, Tetyana
    Zdoryk, Oleksandr
    JOURNAL OF CHEMISTRY AND TECHNOLOGIES, 2023, 31 (02): : 255 - 270
  • [36] Purification and characterization of a trypsin inhibitor from the seeds of Artocarpus heterophyllus Lam.
    Lyu, Junchen
    Liu, Yuan
    An, Tianchen
    Liu, Yujun
    Wang, Manchuriga
    Song, Yanting
    Zheng, Feifei
    Wu, Dan
    Zhang, Yingxia
    Deng, Shiming
    ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2015, 47 (05) : 376 - 382
  • [37] Cloning and characterization of a trypsin inhibitor cDNA from amaranth (Amaranthus hypochondriacus) seeds
    Valdés-Rodríguez, S
    Blanco-Labra, A
    Gutiérrez-Benicio, G
    Boradenenko, A
    Herrera-Estrella, A
    Simpson, J
    PLANT MOLECULAR BIOLOGY, 1999, 41 (01) : 15 - 23
  • [38] EXTRACTION, PURIFICATION, AND CHARACTERIZATION OF A TRYPSIN INHIBITOR FROM COWPEA SEEDS (Vigna unguiculata)
    Wang, Jia
    Li, Xiaona
    Xia, Xunfeng
    Li, Hao
    Liu, Jing
    Li, Qing X.
    Li, Ji
    Xu, Ting
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2014, 44 (01): : 1 - 15
  • [39] Cloning and characterization of a trypsin inhibitor cDNA from amaranth (Amaranthus hypochondriacus) seeds
    Silvia Valdés-Rodríguez
    Alejandro Blanco-Labra
    Glenda Gutiérrez-Benicio
    Anatoli Boradenenko
    Alfredo Herrera-Estrella
    June Simpson
    Plant Molecular Biology, 1999, 41 : 15 - 23
  • [40] ISOLATION OF A TRYPSIN INHIBITOR FROM GUAR
    HOOPER, FG
    COUCH, JR
    FEDERATION PROCEEDINGS, 1971, 30 (02) : A641 - &
12345