A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

被引：9

作者：

Li, Jie ^{[1
]}

Roise, Joachim Justad ^{[1
,2
]}

Zhang, Jingtuo ^{[1
]}

Yang, Jiaying ^{[1
,2
]}

Kerr, D. Lucas ^{[1
,2
]}

Han, Hesong ^{[1
]}

Murthy, Niren ^{[1
,3
]}

机构：

[1] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[3] IGI, 2151 Berkeley Way, Berkeley, CA 94704 USA

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 31期

基金：

美国国家卫生研究院;

关键词：

IN-VIVO DELIVERY; PLASMID DNA; POLYMER; NANOPARTICLES; EFFICACY;

D O I：

10.1039/c9cc00261h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleoprotein by 300% via a combination of fluorescent based enrichment and endosomal disruption.

引用

页码：4562 / 4565

页数：4

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