Micropatterning Extracellular Matrix Proteins on Electrospun Fibrous Substrate Promote Human Mesenchymal Stem Cell Differentiation Toward Neurogenic Lineage

被引:26
|
作者
Li, Huaqiong [1 ,2 ,3 ]
Wen, Feng [3 ]
Chen, Huizhi [3 ]
Pal, Mintu [3 ]
Lai, Yuekun [4 ]
Zhao, Allan Zijian [2 ]
Tan, Lay Poh [3 ]
机构
[1] Wenzhou Med Univ, Inst Biomat & Engn, Wenzhou 325035, Peoples R China
[2] Chinese Acad Sci, Wenzhou Inst Biomat & Engn, 16 Xinsan Rd, Wenzhou 325011, Peoples R China
[3] Nanyang Technol Univ, Sch Mat Sci & Engn, 50 Nanyang Ave, Singapore 639798, Singapore
[4] Soochow Univ, Coll Text & Clothing Engn, Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China
关键词
microcontact printing; electrospinning; biomaterials; stem cell; neurogenesis; MYOGENIC DIFFERENTIATION; NANOSTRUCTURED SURFACES; FILOPODIA; TOPOGRAPHY; EXPRESSION; SCAFFOLDS; FATE; NANOTOPOGRAPHY; INITIATION; NANOSCALE;
D O I
10.1021/acsami.5b09588
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
In this study, hybrid micropatterned grafts constructed via a combination of microcontact printing and electrospinning techniques process were utilized to investigate the influencing of patterning directions on human mesenchymal stem cells (hMSCs) differentiation to desired phenotypes. We found that the stem cells could align and elongate along the direction of the micropattern, where they randomly distributed on nonmicropatterned surfaces. Concomitant with patterning effect of component on stem cell alignment, a commensurate increase on the expression of neural lineage commitment markers, such as microtubule associated protein 2 (MAP2), Nestin, NeuroD1, and Class III beta-Tubulin, were revealed from mRNA expression by quantitative Real Time PCR (qRT-PCR) and MAP2 expression by immunostaining. In addition, the effect of electrospun fiber orientation on cell behaviors was further examined. An angle of 45 degrees between the direction of micropatterning and orientation of aligned fibers was verified to greatly prompt the outgrowth of filopodia and neurogenesis of hMSCs. This study demonstrates that the significance of hybrid components and electrospun fiber alignment in modulating cellular behavior and neurogenic lineage commitment of hMSCs, suggesting promising application of porous scaffolds with smart component and topography engineering in clinical regenerative medicine.
引用
收藏
页码:563 / 573
页数:11
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