THE PRESENCE OF IL-8 GENE POLYMORPHISM AT (-251A/T) AND (-396T/G) POSITION IS RELATED WITH SUSCEPTIBILITY TO PERIODONTITIS DEVELOPMENT

Background: Periodontitis is a chronic inflammatory disruption of the supportive periodontal tissue. It is known that the chronic periodontitis is initiated by the increased level of specific bacteria, but the progression of the disease is defined by characteristics of the host response to the bacterial load. There is evidence that individual genetic, systemic and environmental risk factors can influence the course of the disease. Some genes and intergenic relations are in connection with the modification of the periodontal disease, and so they change the clinical course, the severity and the prognosis of the decease. Purpose: The aim of the current investigation is to establish the presence of IL-8 gene polymorphism and serum levels to IL- 8 in patients with chronic periodontitis. Material and methods: In the recent study, 35 patients (24 females and 11 males) are included with diagnosis moderate to severe periodontitis. Periodontal important parameters such as PD, CAL and Bone loss are included. The level of IL- 8 in a patient's serum is tested by ELISA (enzyme-linked immunosorbent assay). The presence of IL- 8 polymorphism in loci rs4073 (-251A/T) and rs2227307 (-396T/G) is established by polymerase chain reaction (PCR). Results: The analysis of the current assay recognized a statistically significant correlation between: HI and PBI-severity and HI and BOP; a significant correlation between PBI- severity and PD average and Bone loss; the strong correlation between BOP and Bone loss; the significant correlation between PD 3- 5mm and PD >= 7mm and CAL average, also a strong correlation between PD >= 7mm and Bone loss. The results have shown that the biggest interrelationship observed between the presence of GT/AT genotype and TT/TT genotype and distribution of PD 5-7 mm and PD >= 7mm (p<0.09). Conclusions: In the recent study have shown that by reason of the heterogeneous results achieved in the group investigated by us we can definitely support the hypothesis that the presence of allele A for rs407 (-251A/T) and allele G for rs2227307 (-396T/G) is strongly related with expression of severe periodontitis.