A. simple method for the. simultaneous determination of guanine, xanthine and hypoxanthine by RP-HPLC with UV detection

被引：0
|
作者：
Majumder, Natasha [1 ]
Chowdhury, Chumki [1 ]
Jana, Tapan Kumar [1 ]
机构：
[1] Univ Calcutta, Dept Marine Sci, Kolkata 700019, India
来源：
JOURNAL OF THE INDIAN CHEMICAL SOCIETY | 2015年 / 92卷 / 09期
关键词：
Xanthine; hypoxanthine; retention factor; RP-HPLC; PRESSURE LIQUID-CHROMATOGRAPHY; PYRIMIDINE-BASES; URIC-ACID; NUCLEOSIDES; PURINE;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A simple, rapid and accurate method for the simultaneous determination of guanine, xanthine and hypoxanthine has been developed. The quantitative determination of these bases was accomplished by reverse phase high-performance liquid chromatography (RP-HPLC) with UV detection based on their different retention time without chemical suppression. Separation in the order of guanine, hypoxanthine and xanthine were obtained on ODS hypersil column using mobile phase containing 1% ACN in acetic acid (50 mM), ammonium acetate (85 mM) and 0.5% methanol at 0.5 ml min(-1) flow rate. A portion of the natural DNA isolated from Avicennia marina was spiked with known amount of hypoxanthine and its concentration was found to be 1.4 mu M.
引用
收藏
页码：1381 / 1385
页数：5
相关论文
共 50 条
  • [21] Quantitative Determination of Ciprofibrate in Tablets by Derivative UV Spectroscopy and RP-HPLC Method
    Jain, P. S.
    Jivani, H. N.
    Khatal, R. N.
    Surana, S. J.
    INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2012, 74 (02) : 168 - U1171
  • [22] Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC
    Tian, D. F.
    Tian, X. L.
    Tian, T.
    Wang, Z. Y.
    Mo, F. K.
    INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2008, 70 (03) : 372 - U7
  • [23] Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC
    Yadav, Akhilesh K.
    Manika, N.
    Bagchi, Guru D.
    Gupta, Madan M.
    MEDICINAL CHEMISTRY RESEARCH, 2013, 22 (05) : 2222 - 2227
  • [24] Simultaneous Determination of Sixteen Hormones in Cosmetics by RP-HPLC
    Wang Chao
    Ma Qiang
    Wang Xing
    CHINESE JOURNAL OF CHROMATOGRAPHY, 2006, 24 (06) : 654 - 655
  • [25] Simultaneous determination of flavonoids in Oroxylum indicum by RP-HPLC
    Akhilesh K. Yadav
    N. Manika
    Guru D. Bagchi
    Madan M. Gupta
    Medicinal Chemistry Research, 2013, 22 : 2222 - 2227
  • [26] Simultaneous determination of cefdinir and cefixime in human plasma by RP-HPLC/UV detection method: Method development, optimization, validation, and its application to a pharmacokinetic study
    Khan, Abbas
    Iqbal, Zafar
    Khan, Muhammad Imran
    Javed, Khalid
    Khan, Abad
    Ahmad, Lateef
    Shah, Yasar
    Nasir, Fazli
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (24): : 2423 - 2429
  • [27] A simple derivatization RP-HPLC method for the simultaneous determination of zineb and hexaconazole in pesticide formulation using a PDA detector
    Patil, Vilas K.
    Dhande, Nilesh D.
    Petha, Narendra H.
    Narkhede, Hemant P.
    ANALYTICAL METHODS, 2021, 13 (35) : 3930 - 3939
  • [28] Validated RP-HPLC Method for Simultaneous Determination of Telmisartan and Hydrochlorothiazide in Pharmaceutical Formulation
    Megala, K.
    Chenthilnathan, A.
    Babu, A. Sathish
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND ALLIED SCIENCES, 2014, 3 (03): : 33 - 40
  • [29] A NEW VALIDATED METHOD FOR THE SIMULTANEOUS DETERMINATION OF A SERIES OF EIGHT BARBITURATES BY RP-HPLC
    Shabir, Ghulam A.
    Bradshaw, Tony K.
    Arain, Shafique A.
    Shar, Ghulam Qadir
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2010, 33 (01) : 61 - 71
  • [30] Validated RP-HPLC method for simultaneous determination of telmisartan and hydrochlorothiazide in pharmaceutical formulation
    Bhat, Leena R.
    Godge, Rahul K.
    Vora, Asfak T.
    Damle, Mrinalini C.
    JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2007, 30 (20) : 3059 - 3067
12345