A. simple method for the. simultaneous determination of guanine, xanthine and hypoxanthine by RP-HPLC with UV detection

被引：0
|
作者：
Majumder, Natasha [1 ]
Chowdhury, Chumki [1 ]
Jana, Tapan Kumar [1 ]
机构：
[1] Univ Calcutta, Dept Marine Sci, Kolkata 700019, India
来源：
JOURNAL OF THE INDIAN CHEMICAL SOCIETY | 2015年 / 92卷 / 09期
关键词：
Xanthine; hypoxanthine; retention factor; RP-HPLC; PRESSURE LIQUID-CHROMATOGRAPHY; PYRIMIDINE-BASES; URIC-ACID; NUCLEOSIDES; PURINE;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A simple, rapid and accurate method for the simultaneous determination of guanine, xanthine and hypoxanthine has been developed. The quantitative determination of these bases was accomplished by reverse phase high-performance liquid chromatography (RP-HPLC) with UV detection based on their different retention time without chemical suppression. Separation in the order of guanine, hypoxanthine and xanthine were obtained on ODS hypersil column using mobile phase containing 1% ACN in acetic acid (50 mM), ammonium acetate (85 mM) and 0.5% methanol at 0.5 ml min(-1) flow rate. A portion of the natural DNA isolated from Avicennia marina was spiked with known amount of hypoxanthine and its concentration was found to be 1.4 mu M.
引用
收藏
页码：1381 / 1385
页数：5
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