Increased VLDL-TG Fatty Acid Storage in Skeletal Muscle in Men With Type 2 Diabetes

被引：12
|
作者：
Andersen, Iben R. [1 ,2 ]
Sondergaard, Esben [1 ,2 ,5 ,6 ]
Sorensen, Lars P. [1 ,2 ]
Nellemann, Birgitte [1 ,2 ]
Gormsen, Lars C. [3 ,4 ]
Jensen, Michael D. [5 ]
Nielsen, Soren [1 ,2 ]
机构：
[1] Aarhus Univ Hosp, Dept Endocrinol, DK-8000 Aarhus, Denmark
[2] Aarhus Univ Hosp, Dept Internal Med, DK-8000 Aarhus, Denmark
[3] Aarhus Univ Hosp, Dept Nucl Med, DK-8000 Aarhus, Denmark
[4] Aarhus Univ Hosp, PET Ctr, DK-8000 Aarhus, Denmark
[5] Mayo Clin, Endocrine Res Unit, Rochester, MN 55905 USA
[6] Danish Diabet Acad, DK-5000 Odense, Denmark
来源：
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 2017年 / 102卷 / 03期
基金：
英国医学研究理事会;
关键词：
TISSUE LIPOPROTEIN-LIPASE; ACYL-COA SYNTHETASE; ADIPOSE-TISSUE; ENERGY-EXPENDITURE; VISCERAL FAT; INSULIN; SEX; TRIGLYCERIDES; DETERMINANTS; METABOLISM;
D O I：
10.1210/jc.2016-2979
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Context: Lipoprotein lipase (LPL) activity is considered the rate-limiting step of very-low-densitylipoprotein triglycerides (VLDL-TG) tissue storage, and has been suggested to relate to the development of obesity as well as insulin resistance and type 2 diabetes. Objective: The objective of the study was to assess the relationship between the quantitative storage of VLDL-TG fatty acids and LPL activity and other storage factors in muscle and adipose tissue. In addition, we examine whether such relations were influenced by type 2 diabetes. Design: We recruited 23 men ( 12 with type 2 diabetes, 11 nondiabetic) matched for age and body mass index. Postabsorptive VLDL-TG muscle and subcutaneous adipose tissue ( abdominal and leg) quantitative storage was measured using tissue biopsies in combination with a primed-constant infusion of ex vivo triolein labeled [1-C-14]VLDL-TG and a bolus infusion of ex vivo triolein labeled [ 9,10-H-3]VLDL-TG. Biopsies were analyzed for LPL activity and cellular storage factors. Results: VLDL-TG storage rate was significantly greater in men with type 2 diabetes compared with nondiabetic men in muscle tissue (P = 0.02). We found no significant relationship between VLDL-TG storage rate and LPL activity or other storage factors in muscle or adipose tissue. However, LPL activity correlated with fractional VLDL-TG storage in abdominal fat (P = 0.04). Conclusions: Men with type 2 diabetes have increased VLDL-TG storage in muscle tissue, potentially contributing to increased intramyocellular triglyceride and ectopic lipid deposition. Neithermuscle nor adipose tissue storage rates were related to LPL activity. This argues against LPL as a rate-limiting step in the postabsorptive quantitative storage of VLDL-TG.
引用
收藏
页码：831 / 839
页数：9
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