Simultaneous saccharification and fermentation of cassava to succinic acid by Escherichia coli NZN111

被引：44
|
作者：
Chen, Cuixia [1 ]
Ding, Shaopeng [1 ]
Wang, Dezheng [1 ]
Li, Zhimin [1 ]
Ye, Qin [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2014年 / 163卷
基金：
国家高技术研究发展计划(863计划);
关键词：
Succinic acid; Simultaneous saccharification and fermentation; Cassava; Escherichia coli NZN111; LACTIC-ACID; ETHANOL; CULTURE; STARCH;
D O I：
10.1016/j.biortech.2014.04.020
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In this study, the production of succinic acid from cassava starch and raw cassava instead of glucose by Escherichia coli NZN111 was investigated. During the two-stage fermentation, simultaneous saccharification and fermentation (SSF) was applied in the anaerobic stage. The results showed that both the productivity and specific productivity in the process conducted at 40 degrees C were higher than those in the cultivation conducted at 37 degrees C. The yield of succinic acid based on the amount of added starch reached the highest level 0.86 g/g and cassava starch was almost totally hydrolyzed in the SSF process. With the improved cell density, 127.13 g/L of succinic acid was obtained. When the liquefied crude cassava powder was used directly in SSF, 106.17 g/L of succinic acid was formed. The result showed that crude cassava powder could be another cheap raw material for succinic acid formation. (c) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：100 / 105
页数：6
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