Acinetobacter larvae sp nov., isolated from the larval gut of Omphisa fuscidentalis

A Gram-stain-negative, non-spore-forming, non-motile and aerobic coccobacilli-shaped strain, designated BRTC-1(T), was isolated from the gut of Omphisa fuscidentalis Hampson, which is a larva of a moth and was collected from Xishuangbanna Dai Autonomous Prefecture in China. The isolate was found to grow at NaCl concentrations of 0-5% (w/v) (optimum: 0 %), 10-45 degrees C (optimum: 30-35 degrees C) and pH 5.0-9.0 (optimum: pH 6.0) on tryptic soy agar. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Acinetobacter and was most closely related to Acinetobacter rudis LMG 26107(T), Acinetobacter guillouiae LMG 988(T) and Acinetobacter bereziniae LMG 1003(T) with 96.4, 96.3 and 96.3% sequence similarity, respectively. The comparative sequence analyses of the rpoB and gyrB genes showed that BRTC-1(T) is distant from the species of the genus Acinetobacter with validly published names (<= 84.0 and <= 82.0% similarity, respectively). The average nucleotide identity and digital DNA-DNA hybridization values (<= 77.0 and <= 22.8 %, respectively) between the wholegenome sequence of BRTC-1(T) and those of the known taxa were far below the thresholds used to discriminate bacterial species. The major fatty acids were determined to be C-16 : 0,C- C-18 : 1 omega 9c and C-16 : 1 omega 7c/iso-C-15 : 0 2-OH. The respiratory quinone was Q-9. The polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, five phospholipids and one phosphatidylcholine. Based on its phenotypic and chemotaxonomic characteristics from this study, the isolate is concluded to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter larvae sp. nov. is proposed. The type strain is BRTC-1(T) (= ACCC 19936(T) = JCM 31367(T)).