Transcript profiling of early lateral root initiation

被引:159
|
作者
Himanen, K
Vuylsteke, M
Vanneste, S
Vercruysse, S
Boucheron, E
Alard, P
Chriqui, D
Van Montagu, M
Inzé, D
Beeckman, T
机构
[1] Ghent Univ VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium
[2] Univ Paris 06, F-75252 Paris 05, France
关键词
auxin; cell cycle; microarray;
D O I
10.1073/pnas.0308702101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
At the onset of lateral root initiation in Arabidopsis thaliana, the phytohormone auxin activates xylem pole pericycle cells for asymmetric cell division. However, the molecular events leading from auxin to lateral root initiation are poorly understood, in part because the few responsive cells in the process are embedded in the root and are thus difficult to access. A lateral root induction system, in which most xylem pole pericycle cells were synchronously activated by auxin transport inhibition followed by auxin application, was used for microarray transcript profiling. Of 4,600 genes analyzed, 906 significantly differentially regulated genes were identified that could be grouped into six major clusters. Basically, three major patterns were discerned representing induced, repressed, and transiently expressed genes. Analysis of the coregulated genes, which were specific for each time point, provided new insight into the molecular regulation and signal transduction preceding lateral root initiation in Arabidopsis. The reproducible expression profiles during a time course allowed us to define four stages that precede the cell division in the pericycle. These early stages were characterized by G, cell cycle block, auxin perception, and signal transduction, followed by progression over G(1)/S transition and G(2)/M transition. All these processes took place within 6 h after transfer from N-1-naphthylphthalamic acid to 1-naphthalene acetic acid. These results indicate that this lateral root induction system represents a unique synchronized system that allows the systematic study of the developmental program upstream of the cell cycle activation during lateral root initiation.
引用
收藏
页码:5146 / 5151
页数:6
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