Nanosecond fluorescence lifetime low-cost sensor

被引：0

作者：

Franco, Zulay ^{[1
]}

Sotelo, Felix ^{[2
]}

Gomez-de Pedro, Sara ^{[3
]}

Altabas, Jose A. ^{[2
]}

Puyol, Mar ^{[3
]}

Izquierdo, David ^{[2
,4
]}

Alonso, Julian ^{[3
]}

Garces, Ignacio ^{[2
]}

机构：

[1] Univ Nacl Expt Politecn Antonio Jose Sucre, Ctr Diseno Microelect, Dept Ingn Elect, Vice Rectorado Puerto Or, Venezuela

[2] Univ Zaragoza, Inst Invest Ingn Aragon I3A, Photon Tech Grp, Zaragoza, Spain

[3] Autonomous Univ Barcelona, Sensors & Biosensors Grp, Bellaterra, Spain

[4] Ctr Univ La Defensa, Zaragoza, Spain

来源：

2014 IEEE SENSORS | 2014年

关键词：

Fluorescence lifetime; optical sensor; RF modulation; microfluidic devices;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A novel nanosecond fluorescence lifetime low-cost system have been developed combining the most advanced optoelectronic and RF semiconductor technologies with LTCC (Low Temperature Co-fired Ceramic) microfluidic integrated systems. The lifetime measurement is done by phase-modulation technique, which uses sine-modulated light source and calculates the lifetime measuring the phase between emission and fluorescence. The modulation frequency determines the minimum lifetime, so to measure nanosecond lifetimes, the modulation frequency has to be over 100MHz. The performance of the sensor has been tested with a fluorescent dye. The used dye fluorescence lifetime, 2.44ns, was measured by commercial equipment and matches with the lifetime obtained by our system. In addition, the sensor shows a fast response to variations in the fluorescence lifetime due to fluorescence quenching in the presence of Cu2+.

引用

页码：32 / 35

页数：4

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