Mitochondrial DNA Polymorphism among Populations of Melipona quadrifasciata quadrifasciata Lepeletier (Apidae: Meliponini) from Southern Brazil

被引:1
|
作者
Torres, Rogelio R. [1 ]
Arias, Maria C. [2 ]
Moretto, Geraldo [3 ]
机构
[1] Univ Nacl Colombia, Dept Anim Prod, Bogota, Colombia
[2] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo, Brazil
[3] Univ Reg Blumenau, Dept Ciencias Nat, BR-89010971 Blumenau, SC, Brazil
关键词
mtDNA; PCR-RLFP; stingless bee; haplotype; APIS-MELLIFERA; EVOLUTIONARY; RESTRICTION; HYMENOPTERA;
D O I
10.1590/S1519-566X2009000200007
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The geographical distribution of the Brazilian endemic stingless bee Melipona quadrifasciata quadrifasciata Lepeletier ranges from Rio Grande do Sul to Minas Gerais states. The objective of the present study was to verify mtDNA polymorphisms among samples of M. q. quadrifasciata collected in southern Brazil. Twenty nine colonies from three localities (Blumenau and Mafra/SC and Prudentopolis/PR) were sampled. Seven mtDNA regions were amplified and further digested with 15 restriction enzymes (PCR-RFLP). Five composite haplotypes were identified, with two unique to samples from Prudentopolis and the remaining three to samples from Mafra and/or Blumenau.
引用
收藏
页码:208 / 212
页数:5
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