HELICOBACTER PYLORI INFECTION: A BIOINFORMATIC APPROACH

Helicobacter pylori causative agent of acid peptic disease is a microaerophilic, spiral-shaped, gram-negative bacteria found in the gastric epithelium that may also lead to complications such as chronic gastritis, mucosa-associated lymphoid tissue (MALT) lymphoma, gastroduodenal ulcer and adenocarcinoma in stomach. Plantbioactives have always been potential therapeutics. Usage of modern bioinformatic tools plays a vital role in exploiting the potentials of alternative therapeutic molecules in managing diseases like peptic ulcers and their complications. The in-silico evaluation was carried out using molecular docking of the quorum sensing proteins of H. pylori with the ligand beta-sitosterol obtained from the silver nanoparticles of Acorus calamus L. Among several given quorum sensing proteins the molecular interaction with the ligand beta-sitosterol showed a high binding affinity with DnaA, PhnB, ToxB and Sip proteins. The results obtained from molecular interaction study revealed that the ligand beta-sitosterol will be readily taken up by the organism, thereby facilitating easy inhibition or inactivation of quorum sensing molecules ToxB, DnaA, PhnB, and Sip, making it a novel therapeutic alternative to treat H. pylori infections.