Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides

被引：2

作者：

Khanal, Neelam ^{[1
]}

Masellis, Chiara ^{[2
]}

Kamrath, Michael Z. ^{[2
,3
]}

Clemmer, David E. ^{[1
]}

Rizzo, Thomas R. ^{[2
]}

机构：

[1] Indiana Univ, Dept Chem, 800 East Kirkwood Ave, Bloomington, IN 47405 USA

[2] Ecole Polytech Fed Lausanne, Lab Chim Phys Mol, SB, ISIC, Stn 6, CH-1015 Lausanne, Switzerland

[3] TOFWERK AG, Uttingenstr 22, CH-3600 Thun, Switzerland

来源：

ANALYST | 2018年 / 143卷 / 08期

基金：

瑞士国家科学基金会; 美国国家卫生研究院;

关键词：

ELECTROPHORESIS-MASS SPECTROMETRY; HIGH-RESOLUTION; IRMPD SPECTROSCOPY; GLYCAN ANALYSIS; FUCOSYLATION; FUCOSE; REARRANGEMENTS; GLYCOSYLATION; GLYCOPROTEINS; PREBIOTICS;

D O I：

10.1039/c8an00230d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report here our combination of cryogenic, messenger-tagging, infrared (IR) spectroscopy with ion mobility spectrometry (IMS) and mass spectrometry (MS) as a way to identify and analyze a set of human milk oligosaccharides (HMOs) ranging from trisaccharides to hexasaccharides. The added dimension of IR spectroscopy provides a diagnostic fingerprint in the OH and NH stretching region, which is crucial to identify these oligosaccharides, which are difficult to distinguish by IMS alone. These results extend our previous work in demonstrating the generality of this combined approach for distinguishing subtly different structural and regioisomers of glycans of biologically relevant size.

引用

页码：1846 / 1852

页数：7

共 50 条

[31] Intrinsic Structure and Electronic Spectrum of Deprotonated Biliverdin: Cryogenic Ion Spectroscopy and Ion Mobility
Zagorec-Marks, Wyatt
Dodson, Leah G.
Weis, Patrick
Schneider, Erik K.
Kappes, Manfred M.
Weber, J. Mathias
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2021, 143 (42) : 17778 - 17785
[32] Methods for the quantitation of human milk oligosaccharides in bacterial fermentation by mass spectrometry
Ninonuevo, Milady R.
Ward, Robert E.
LoCascio, Riccardo G.
German, J. Bruce
Freeman, Samara L.
Barboza, Mariana
Mills, David A.
Lebrilla, Carlito B.
ANALYTICAL BIOCHEMISTRY, 2007, 361 (01) : 15 - 23
[33] Glycan analysis by ion mobility-mass spectrometry and gas-phase spectroscopy
Manz, Christian
Pagel, Kevin
CURRENT OPINION IN CHEMICAL BIOLOGY, 2018, 42 : 16 - 24
[34] Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints
Abikhodr, Ali H.
Yatsyna, Vasyl
Ben Faleh, Ahmed
Warnke, Stephan
Rizzo, Thomas R.
ANALYTICAL CHEMISTRY, 2021, 93 (44) : 14730 - 14736
[35] Ammonia analysis by ion mobility spectroscopy
Pfeiffer, R
15TH CONFERENCE ON BIOMETEOROLOGY AND AEROBIOLOGY JOINT WITH THE 16TH INTERNATIONAL CONGRESS ON BIOMETEOROLOGY, 2002, : 166 - 167
[36] Isomeric Oligosaccharides Analyses Using Negative-ion Electrospray Ionization Ion Mobility Spectrometry Combined with Collision-induced Dissociation MS/MS
Tohru Yamagaki
Akihiro Sato
Analytical Sciences, 2009, 25 : 985 - 988
[37] Lipid analysis by ion mobility spectrometry combined with mass spectrometry: A brief update with a perspective on applications in the clinical laboratory
Dubland, Joshua A.
JOURNAL OF MASS SPECTROMETRY AND ADVANCES IN THE CLINICAL LAB, 2022, 23 : 7 - 13
[38] Miniaturized Ultra High Field Asymmetric Waveform Ion Mobility Spectrometry Combined with Mass Spectrometry for Peptide Analysis
Brown, L. J.
Toutoungi, D. E.
Devenport, N. A.
Reynolds, J. C.
Kaur-Atwal, G.
Boyle, P.
Creaser, C. S.
ANALYTICAL CHEMISTRY, 2010, 82 (23) : 9827 - 9834
[39] Isomeric Oligosaccharides Analyses Using Negative-ion Electrospray Ionization Ion Mobility Spectrometry Combined with Collision-induced Dissociation MS/MS
Yamagaki, Tohru
Sato, Akihiro
ANALYTICAL SCIENCES, 2009, 25 (08) : 985 - 988
[40] Human Cerebellum Gangliosides: A Comprehensive Analysis by Ion Mobility Tandem Mass Spectrometry
Biricioiu, Maria Roxana
Sarbu, Mirela
Ica, Raluca
Vukelic, Zeljka
Clemmer, David E.
Zamfir, Alina D.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2024, 35 (04) : 683 - 695

← 1 2 3 4 5 →