In vitro bud culture of Kinnow tree

Tissues from field grown trees have contamination problems in In vitro cultures. Two hundred explants were tried in each sterilizing chemical viz., HgCl2 and NaOCl. Both the chemicals were effective in making clean explants growth as 62 (31%) and 67 (33%) respectively. The dead explants were 25 and 23. The buds sprouted and upon subculturing, shoots were grown in MS medium supplemented with BA (2 mg/l), GA (1 mg/l), proline (5 mg/l). The developed shoots rooted in half strength medium with addition of IBA (2 mg/1). Our objective was to obtain rooted shoots. Although there were callus formation, leaf growth but no true shoot elongation. The rooted shoots were grafted on young rough lemon seedlings. The overall procedure is lengthy but plants can be obtained from In vitro bud culture of field grown Kinnow tree.