Synthesis of Nano-Hydroxyapatite of Different Morphologies and Their Cellular Compatibility

Nano-hydroxyapatite crystals of different morphologies were synthesized by adding two types of amino acids (glycine and arginine) under hydrothermal conditions. The XRD, FTIR, and TEM characterizations of samples showed that the final product was pure hydroxyapatite with high crystallinity. Organic small-molecule amino acids exhibited a significant inhibitory effect on crystal growth during the synthesis process. This regulatory effect is related to the side chains of amino acids. The results of co-culturing with bone mesenchymal stem cells showed that the cell compatibility of nanoparticles differs based on their morphologies. The results of this study are significant for the fabrication of nano-hydroxyapatite with tunable morphology, which can have applications in the fields of bone repair and drug loading. Nano-hydroxyapatite with different morphology and size was prepared by hydrothermal method under the regulation of amino acids. The side chain composition of amino acids plays an important role in the process of crystal growth. The result of CCK-8 assay showed that the particles with smaller particle size showed better cytocompatibility.