Identification of CD4+ T-cell epitopes on iron-regulated surface determinant B of Staphylococcus aureus

被引:6
|
作者
Yu, Simiao [1 ]
Zhang, Hua [1 ]
Yao, Di [2 ]
Liu, Wei [1 ]
Wang, Xintong [1 ]
Chen, Xiaoting [2 ]
Wei, Yuhua [2 ]
Zhang, Zhenghai [1 ]
Wang, Jiannan [1 ]
Yu, Liquan [1 ]
Sun, Hunan [1 ]
Wu, Zhijun [1 ]
Yu, Yongzhong [1 ]
Song, Baifen [1 ]
Ma, Jinzhu [1 ]
Tong, Chunyu [1 ]
Cui, Yudong [1 ,2 ]
机构
[1] HeiLongliang BaYi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Peoples R China
[2] HeiLongliang BaYi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Peoples R China
基金
中国国家自然科学基金;
关键词
Staphylococcus aureus; IsdB protein; T-cell epitope; GAPC PROTEIN; HEME-IRON; HEMOGLOBIN; PREDICTION; BINDING; ISDB; ANTIBODY; RECEPTOR; SYSTEM;
D O I
10.1016/j.micpath.2015.09.006
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Iron-regulated surface determinant B (IsdB) of Staphylococcus aureus (S. aureus) is a highly conserved surface protein that can induce protective CD4(+) T-cell immune response. A pivotal role of CD4(+) T-cells in effective immunity against S. aureus infection has been proved, but CD4(+) T-cell epitopes on the S. aureus IsdB have not been well identified. In this study, MHC binding assay was firstly used to predict CD4 T-cell epitopes on S. aureus IsdB protein, and six peptides were synthesized to validate the probable epitopes. Two novel IsdB CD4(+) T-cell epitopes, P1 (residues 159-178) and P4 (residues 287-306), were for the first time identified using CD4(+) T-cells obtained from IsdB-immunized C57BL/6 (H-2(b)) and BALB/c (H-2(d)) mice spleen based on cell proliferation and cytokines response. The results showed that P1 and P4 emulsified in Freund's adjuvant (FA) induced much higher cell proliferation compared with PBS emulsified in FA. CD4(+) T-cells stimulated with peptides P1 and P4 secreted significantly higher levels of IFN-gamma and IL-17A. However, the level of the cytokine IL-4 almost remained unchanged, suggesting that P1 and P4 preferentially elicited polarized Th1-type responses. In addition, BALB/c mice just respond to P4 not P1, while C57BL/6 mice respond to P1 not P4, implying that epitope P1 and P4 were determined as H-2(b) and H-2(d) restricted epitope, respectively. Taken together, our data may provide an explanation of the IsdB-induced protection against S. aureus and highlight the possibility of developing the epitope-based vaccine against the S. aureus. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:108 / 113
页数:6
相关论文
共 50 条
  • [21] CD4+ T-CELL EPITOPES IN HIV-1 PROTEINS
    SILICIANO, RF
    CHEMICAL IMMUNOLOGY, 1993, 56 : 127 - 149
  • [22] Direct hemin transfer from IsdA to IsdC in the iron-regulated surface determinant (Isd) heme acquisition system of Staphylococcus aureus
    Liu, Mengyao
    Tanaka, Wesley N.
    Zhu, Hui
    Xie, Gang
    Dooley, David M.
    Lei, Benfang
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (11) : 6668 - 6676
  • [23] Identification of CD4+ T cell epitopes of Taenia solium paramyosin
    López-Moreno, HS
    Correa, D
    Laclette, JP
    Ortiz-Navarrete, VF
    PARASITE IMMUNOLOGY, 2003, 25 (10) : 513 - 516
  • [24] An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis
    Mazmanian, SK
    Ton-That, H
    Su, K
    Schneewind, O
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) : 2293 - 2298
  • [25] Force Nanoscopy Demonstrates Stress-Activated Adhesion between Staphylococcus aureus Iron-Regulated Surface Determinant Protein B and Host Toll-like Receptor 4
    Paiva, Telmo O.
    Speziale, Pietro
    Dufrene, Yves F.
    ACS NANO, 2024, 19 (01) : 989 - 998
  • [26] Conservation, surface exposure, and in vivo expression of the Frp family of iron-regulated cell wall proteins in Staphylococcus aureus
    Morrissey, JA
    Cockayne, A
    Hammacott, J
    Bishop, K
    Denman-Johnson, A
    Hill, PJ
    Williams, P
    INFECTION AND IMMUNITY, 2002, 70 (05) : 2399 - 2407
  • [27] The iron-regulated surface proteins IsdA, IsdB, and IsdH are not required for heme iron utilization in Staphylococcus aureus
    Hurd, Alexander F.
    Garcia-Lara, Jorge
    Rauter, Yvonne
    Cartron, Michael
    Mohamed, Ramlan
    Foster, Simon J.
    FEMS MICROBIOLOGY LETTERS, 2012, 329 (01) : 93 - 100
  • [28] The identification of CD4+ T cell epitopes with dedicated synthetic peptide libraries
    Hiemstra, HS
    Duinkerken, G
    Benckhuijsen, WE
    Amons, R
    deVries, RRP
    Roep, BO
    Drijfhout, JW
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (19) : 10313 - 10318
  • [29] Identification and Characterization of CD4+ T Cell Epitopes after Shingrix Vaccination
    Voic, Hannah
    de Vries, Rory D.
    Sidney, John
    Rubiro, Paul
    Moore, Erin
    Phillips, Elizabeth
    Mallal, Simon
    Schwan, Brittany
    Weiskopf, Daniela
    Sette, Alessandro
    Grifoni, Alba
    JOURNAL OF VIROLOGY, 2020, 94 (24)
  • [30] Identification of Cross-Reactive Norovirus CD4+ T Cell Epitopes
    LoBue, Anna D.
    Lindesmith, Lisa C.
    Baric, Ralph S.
    JOURNAL OF VIROLOGY, 2010, 84 (17) : 8530 - 8538