Total internal reflection-based module for fluorescence and absorbance detection

被引：6

作者：

Verschooten, Tom ^{[1
]}

Ottevaere, Heidi ^{[1
]}

Vervaeke, Michael ^{[1
]}

Van Erps, Juergen ^{[1
]}

Thienpont, Hugo ^{[1
]}

机构：

[1] Vrije Univ Brussel, Dept Appl Phys & Photon, Brussels Photon Team, B-1050 Brussels, Belgium

来源：

JOURNAL OF MICRO-NANOLITHOGRAPHY MEMS AND MOEMS | 2014年 / 13卷 / 03期

关键词：

micro-optics; lab-on-a-chip; absorbance detection; fluorescence detection; rapid prototyping; deep proton writing; DETECTION SYSTEM; MICROCHIP;

D O I：

10.1117/1.JMM.13.3.033001

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present a miniaturized polymer-based micro-optical detection unit for ultraviolet and visible laser-induced fluorescence (LIF) and absorbance (ABS) analysis with an interaction length of 3 mm. We use non-sequential optical ray tracing simulations to model the system and to optimize its performance with respect to optical efficiency and system complexity. The design features a compact optical system combining total internal reflection (TIR) mirrors and refractive optics. The detection module is prototyped with deep proton writing in 2-mm-thick polymethylmethacrylate and quantitatively characterized using a three-dimensional coordinate measurement machine. We demonstrate the proof-of-concept of this TIR mirror-based module for coumarin 480 obtaining limits of detection of 50 pM and 500 nM for LIF and ABS, respectively. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：9

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