Endothelial expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 is suppressed by postbypass plasma containing increased soluble intercellular adhesion molecule 1 and vascular cell adhesion molecule 1

Objective: Endothelial cell dysfunction has been implicated in the inflammatory response to cardiopulmonary bypass, and the upregulation of endothelial cell expression of adhesion molecules might promote leukocyte extravasation in vivo. Soluble endothelial cell adhesion molecules are increased after bypass. The aim of this study was to investigate the relationship between endothelial Cell-surface expression of adhesion molecules and their concentration in plasma after coronary artery bypass grafting. Methods: Ten patients undergoing coronary artery bypass with cardiopulmonary bypass had 5 plasma sample, taken Lit defined internals before, during and after cardiopulmonary bypass. plamsa was incubated with human umbilical vein endothelial cell monolayers. and expression of E-selectin, intercellular adhesion molecule 1. and vascular Cell adhesion molecule I on the surface of human umbilical vein endothelial cell monolayers was measured by means of enzyme-linked immunosorbent assay. Plasma soluble adhesion molecules, C-reactive protein, interleukin 8. interleukin 10, transforming growth factor beta1, and neutrophil Counts were determined for each patient. Results: Markers typical of acute inflammation (ie. interleukin 8, neutrophils. and C-reactive protein) were all increased after bypass. Soluble plasma intercellular and vascular cell adhesion molecule I (but not E-selectin) were increased after bypass. However. endothelial cell expression of vascular cell adhesion molecule I and intercellular adhesion Molecule I (hut not E-selectin) were significantly decreased h exposure to postbypass plasma. Additionally, postbypass plasma inhibited interleukin 1beta-stimulated endothelial cell expression Of vascular cell and intercellular adhesion molecule 1. Interleukin 10 and transforming growth factor beta1, both of which are known to inhibit endothelial cell adhesion molecule expression. were respectively increased 10-fold and 3-fold (p < .05) after bypass, Conclusions: Despite containing increased soluble intercellular and vascular cell adhesion molecule 1, postbypass plasma inhibits endothelial cell expression of intercellular and vascular cell adhesion Molecule 1. upregulated vascular expression of adhesion molecules might not he essential for endothelial activation after bypass.