A study of aspartyl proteases using intramolecularly quenched fluorogenic peptide substrates

被引：0

作者：

Filippova, IY ^{[1
]}

Lysogorskaya, EN ^{[1
]}

Lavrenova, GI ^{[1
]}

Oksenoit, ES ^{[1
]}

Suvorov, LI ^{[1
]}

Starovoitova, VV ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119899, Russia

来源：

BIOORGANICHESKAYA KHIMIYA | 2000年 / 26卷 / 03期

关键词：

aspartyl proteases; fluorogenic peptide substrates;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A series of fluorogenic tetra-, penta-, and hexapeptide substrates of the general structure Abz-X-Phe-Phe-Y-Ded (or -pNa in place of -Ded), where X = Ala, Ala-Ala, or Val-Ala and Y = -, Ala, or Ala-Ala, were proposed, Kinetic parameters of hydrolysis of these substrates by pepsin, cathepsin D, human gastricsin, pig pepsin, calf chymosin, and aspergillopepsin A were determined. The compounds synthesized proved to be effective substrates for aspartyl proteases of diverse origins.

引用

页码：192 / 196

页数：5

共 50 条

[31] 4-METHYLUMBELLIFERYL ESTERS AS FLUOROGENIC SUBSTRATES FOR PROTEASES
GRAY, CJ
DSILVA, CJSJ
BOUKOUVALAS, J
BARKER, SA
ENZYME AND MICROBIAL TECHNOLOGY, 1983, 5 (02) : 137 - 142
[32] A GENERAL-METHOD FOR THE PREPARATION OF INTERNALLY QUENCHED FLUOROGENIC PROTEASE SUBSTRATES USING SOLID-PHASE PEPTIDE-SYNTHESIS
MAGGIORA, LL
SMITH, CW
ZHANG, ZY
JOURNAL OF MEDICINAL CHEMISTRY, 1992, 35 (21) : 3727 - 3730
[33] PORTION-MIXING PEPTIDE LIBRARIES OF QUENCHED FLUOROGENIC SUBSTRATES FOR COMPLETE SUBSITE MAPPING OF ENDOPROTEASE SPECIFICITY
MELDAL, M
SVENDSEN, I
BREDDAM, K
AUZANNEAU, FI
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) : 3314 - 3318
[34] KINETIC-STUDIES OF THE ASPARTYL PROTEASES WITH VARIATION IN THE P3 RESIDUE OF CHROMOPHORIC PEPTIDE-SUBSTRATES
DUNN, BM
KAY, J
JIMENEZ, M
VALLER, MJ
RAO, JK
PARTEN, BF
BIOPHYSICAL JOURNAL, 1984, 45 (02) : A16 - A16
[35] Characterization of a prolyl endopeptidase (kininase) from human urine using fluorogenic quenched substrates
Quinto, BMR
Juliano, MA
Hirata, I
Carmona, AK
Juliano, L
Casarini, DE
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2000, 32 (11-12): : 1161 - 1172
[36] Selection of chromogenic and fluorogenic substrates of neutrophil serine proteases using combinatorial chemistry approach
Lesner, Adam
Magdalena, Wysocka
Spichalska, Bogena
Mackiewicz, Lucyna
Legowska, Anna
Guzow, Katarzyna
Wiczk, Wiesaw
Rolka, Krzysztof
JOURNAL OF PEPTIDE SCIENCE, 2008, 14 (08) : 88 - 88
[37] Assembly and selective "in synthesis" labeling of quenched fluorogenic protease substrates
Chersi, Alberto
Ferracuti, Silvia
Falasca, Giuliana
Butler, Richard H.
Fruci, Doriana
ANALYTICAL BIOCHEMISTRY, 2006, 357 (02) : 194 - 199
[38] Analysis of the subsite specificity of rat insulysin using fluorogenic peptide substrates
Song, ES
Mukherjee, A
Juliano, MA
St Pyrek, J
Goodman, JP
Juliano, L
Hersh, LB
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (02) : 1152 - 1155
[39] Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
Szabelski, M
Rogiewicz, M
Wiczk, W
ANALYTICAL BIOCHEMISTRY, 2005, 342 (01) : 20 - 27
[40] Synthesis and hydrolysis by cysteine and serine proteases of short internally quenched fluorogenic peptides
Melo, RL
Alves, LC
Del Nery, E
Juliano, L
Juliano, MA
ANALYTICAL BIOCHEMISTRY, 2001, 293 (01) : 71 - 77

← 1 2 3 4 5 →