Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogene

被引：42
|
作者：
Soleimaninejad, Hamid [1 ]
Chen, Moore Z. [1 ]
Lou, Xiaoding [2 ]
Smith, Trevor A. [1 ]
Hong, Yuning [1 ,3 ]
机构：
[1] Univ Melbourne, Sch Chem, Parkville, Vic 3010, Australia
[2] Huazhong Univ Sci & Technol, Minist Educ, Sch Chem & Chem Engn, Key Lab Mat Chem Energy Convers & Storage,Hubei K, Wuhan 430074, Peoples R China
[3] La Trobe Univ, La Trobe Inst Mol Sci, Dept Chem & Phys, Melbourne, Australia
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 19期
关键词：
AGGREGATION-INDUCED EMISSION; LIVING CELLS; PROTEIN DIFFUSION; IN-VIVO; CONFINEMENT; MICROSCOPY;
D O I：
10.1039/c6cc09916e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.
引用
收藏
页码：2874 / 2877
页数：4
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