SEQUENCE-BASED AND MONOCLONAL ANTIBODY TYPING OF LEGIONELLA PNEUMOPHILA ISOLATED FROM PATIENTS IN PORTUGAL DURING 1987-2008

被引：0
|
作者：
Chasqueira, M. J. [1 ]
Rodrigues, L. [1 ]
Nascimento, M. [2 ]
Marques, T. [1 ,2 ]
机构：
[1] Univ Nova Lisboa, Dept Microbiol, Chron Dis Res Ctr CEDOC, FCM,Fac Med Sci, Lisbon, Portugal
[2] Santa Cruz Hosp HSC CHLO, Microbiol Lab, Carnaxide, Portugal
来源：
EUROSURVEILLANCE | 2009年 / 14卷 / 28期
关键词：
LEGIONNAIRES-DISEASE; SEROGROUP-1; STRAINS; SUBGROUPS; OUTBREAK; SCHEME;
D O I：
暂无
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The monoclonal antibodies and the sequence-based typing (SBT) are two methodologies widely used to characterise Legionella pneumophila strains serogroup 1 (sg1). In this study, we analysed the clinical strains received in two Portuguese laboratories since 1987, including the strains isolated in Portugal during the four years of the surveillance scheme for Legionnaires' disease implemented in 2004. In total, 63 clinical isolates of L. pneumophila sg1 were differentiated by SBT into 19 different sequence types. Ten of them were new in the SBT database of the European Working Group for Legionella Infections (EWGLI). As a result of the combination of the two methodologies, these strains were discriminated into 25 different profiles. This study enabled, for the first time in Portugal, not only to characterise the L. pneumophila sg1 clinical isolates, but also to create a database of Portuguese profiles for use in epidemiological surveillance efforts.
引用
收藏
页码：11 / 14
页数：4
相关论文
共 50 条
  • [31] Epidemiological Investigation of Legionella pneumophila Serogroup 2 to 14 Isolates from Water Samples by Amplified Fragment Length Polymorphism and Sequence-Based Typing and Detection of Virulence Traits
    Katsiaflaka, Anna
    Pournaras, Spyros
    Kristo, Ioulia
    Mouchtouri, Varvara A.
    Kyritsi, Maria
    Velonakis, Emmanuel
    Vatopoulos, Alkiviadis C.
    Hadjichristodoulou, Christos
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2016, 82 (20) : 6102 - 6108
  • [32] HLA genes in Madeira Island (Portugal) inferred from sequence-based typing:: Footprints from different origins
    Spínola, H
    Bruges-Armas, J
    Mora, MG
    Middleton, D
    Brehm, A
    MOLECULAR IMMUNOLOGY, 2006, 43 (10) : 1726 - 1728
  • [33] Application of Legionella pneumophila-specific quantitative real-time PCR combined with direct amplification and sequence-based typing in the diagnosis and epidemiological investigation of Legionnaires’ disease
    M. Mentasti
    N. K. Fry
    B. Afshar
    C. Palepou-Foxley
    F. C. Naik
    T. G. Harrison
    European Journal of Clinical Microbiology & Infectious Diseases, 2012, 31 : 2017 - 2028
  • [34] Molecular typing of Candida albicans strains isolated from denture wearers by repetitive sequence-based PCR
    O. Abaci
    A. Haliki-Uztan
    B. Ozturk
    S. Toksavul
    M. Ulusoy
    H. Boyacioglu
    European Journal of Clinical Microbiology & Infectious Diseases, 2011, 30 : 141 - 149
  • [35] Molecular typing of Candida albicans strains isolated from denture wearers by repetitive sequence-based PCR
    Abaci, O.
    Haliki-Uztan, A.
    Ozturk, B.
    Toksavul, S.
    Ulusoy, M.
    Boyacioglu, H.
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2011, 30 (02) : 141 - 149
  • [36] Application of Legionella pneumophila-specific quantitative real-time PCR combined with direct amplification and sequence-based typing in the diagnosis and epidemiological investigation of Legionnaires' disease
    Mentasti, M.
    Fry, N. K.
    Afshar, B.
    Palepou-Foxley, C.
    Naik, F. C.
    Harrison, T. G.
    EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2012, 31 (08) : 2017 - 2028
  • [37] Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains
    Ratzow, Sandra
    Gaia, Valeria
    Helbig, Juergen Herbert
    Fry, Norman K.
    Lueck, Paul Christian
    JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) : 1965 - 1968
  • [38] Molecular typing of Stenotrophomonas maltophilia from patients with cystic fibrosis by repetitive sequence-based PCR
    Goncalves-Vidigal, P.
    Buer, J.
    Rath, P. -M.
    Steinmann, J.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 301 : 1 - 1
  • [39] LEGIONELLAE ISOLATED FROM CLINICAL AND ENVIRONMENTAL-SAMPLES IN SPAIN (1983-90) - MONOCLONAL TYPING OF LEGIONELLA-PNEUMOPHILA SEROGROUP-1 ISOLATES
    PELAZ, C
    GARCIA, L
    MARTINBOURGON, C
    EPIDEMIOLOGY AND INFECTION, 1992, 108 (03): : 397 - 402
  • [40] Does better identification of the Legionella pneumophila serogroup 1 strains by Sequence-Based Typing (SBT) allow for the implementation of more effective contamination control strategies and more targeted intervention measures?
    Ditommaso, Savina
    Giacomuzzi, Monica
    Rivera, Susan R. Arauco
    Zotti, Carla M.
    MICROCHEMICAL JOURNAL, 2014, 115 : 126 - 129
12345