The G-protein-coupled CCK2 receptor associates with phospholipase Cγl

被引：13

作者：

Arnould, M

Tassa, A

Ferrand, A

Archer, E

Estève, JP

Pénalba, V

Portolan, G

Escherich, A

Moroder, L

Fourmy, D

Seva, C

Dufresne, M

机构：

[1] CHU Rangueil, IFR31, INSERM, U531,Inst Louis Bugnard, Toulouse, France

[2] CHU Rangueil, IFR31, Funct Proteom Facil, Toulouse, France

[3] CHU Rangueil, IFR31, Toulouse, France

[4] Max Planck Inst Biochem, Martinsried, Germany

来源：

FEBS LETTERS | 2004年 / 568卷 / 1-3期

关键词：

G-protein-coupled receptor; phospholipase C; CCK2; receptor; precancerous condition; surface plasmon resonance; gastrin;

D O I：

10.1016/j.febslet.2004.05.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In ElasCCK2 transgenic mice expressing cholecystokinin (CCK2) receptor in acinar cells, pancreatic phenotypic alterations and preneoplastic lesions are observed. We determined whether activation of phospholipase C gammal (PLCgamma1), known to contribute to the tumorigenesis pathophysiology, could take place as a new signaling pathway induced by the CCK2 receptor. Overexpression and activation of the PLCgamma1 in response to gastrin was observed in acinar cells. The possibility that the C-terminal tyrosine 438 of the CCK2 receptor associates with the SH2 domains of PLCgamma1 was examined. A specific interaction was demonstrated using surface plasmon resonance, confirmed in a cellular system and by molecular modeling. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：89 / 93

页数：5

共 26 条

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[12] IDENTIFICATION OF A REGION OF THE N-TERMINAL OF THE HUMAN CCKA RECEPTOR ESSENTIAL FOR THE HIGH-AFFINITY INTERACTION WITH AGONIST CCK [J].