The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes

被引：11
|
作者：
Tettey, JNA
Skellern, GG
Midgley, JM
Grant, MH
Pitt, AR
机构：
[1] Univ Strathclyde, Dept Pharmaceut Sci, Glasgow G4 0NR, Lanark, Scotland
[2] Univ Strathclyde, Bioengn Unit, Glasgow G4 0NW, Lanark, Scotland
[3] Univ Strathclyde, Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland
来源：
CHEMICO-BIOLOGICAL INTERACTIONS | 1999年 / 123卷 / 02期
关键词：
ethidium bromide; metabolism; hepatocytes; N-glucuronidation; inducing agents;
D O I：
10.1016/S0009-2797(99)00127-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The metabolism of ethidium bromide by isolated rat hepatocytes is significantly enhanced by pre-treatment of animals with phenobarbitone (PB) and 3-methylcholanthrene (3-MC). Pre-treatment with PB and 3-MC results in a 2.5- and 1.5-fold increase, respectively in the amount of the principal metabolite, ethidium 8-N-glucuronide, compared with that formed by hepatocytes from untreated rats. The formation of ethidium 3-N-glucuronide is not enhanced by pre-treatment with either PB or 3-MC. Two new metabolites, hydroxyethidium glucuronide and a transient unidentified species, have been detected by HPLC and are formed only by hepatocytes from animals pre-treated with 3-MC. (C) 1999 Elsevier Science ireland Ltd. All rights reserved.
引用
收藏
页码：105 / 115
页数：11
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